|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD172a, clone BL1H7 is specific for porcine CD172a, a member of the signal regulatory protein (SIRP) family (Alvarezet al.2000). Clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haversonet al.2001;Thackeret al.2001).CD172a is expressed on monocyte derived dendritic cells (MoDCs) (Facciet al.2010) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci;Jeonget al.2010). Clone BL1H7 immunoprecipitated a single band of ~90-110 kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions (Alvarezet al.2000).Mouse anti-Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues (Domenechet al.2003).Aberrant expression of CD172a has been noted on porcine leukemias (Siposet al.2006) with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC (Chamorroet al.2005).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD172a Antibody, clone BL1H7 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Alvarez, B. et al. (2000) A porcine cell surface receptor identified by monoclonal antibodies to SWC3 is a member of the signal regulatory protein family and associates with protein-tyrosine phosphatase SHP-1.Tissue Antigens. 55 (4): 342-51. 2. Bullido, R. et al. (2000) Induction of aggregation in porcine lymphoid cells by antibodies to CD46.Vet Immunol Immunopathol. 73 (1): 73-81. 3. Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.Vet Immunol Immunopathol. 89 (1-2): 91-8. 4. Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.Vet Immunol Immunopathol. 94 (1-2): 77-81. 5. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.Clin Vaccine Immunol. 17 (4): 503-12. 6. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.Vet Res. 42: 9. 7. Moreno, S. et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.Vet Res. 41: 76. 8. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.Immunology. 129: 396-405. 9. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.Vet Immunol Immunopathol. 104: 131-44. 11. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.J Immunol. 188: 3382-94. 12. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.Cell Biol Int. 36 (12): 1233-8. 13. Robinson, S.R. et al. (2015) Broadly neutralizing antibodies against the rapidly evolving porcine reproductive and respiratory syndrome virus.Virus Res. 203: 56-65. 14. Fraile, L. et al. (2012) Immunomodulatory properties of beta-sitosterol in pig immune responses.Int Immunopharmacol. 13 (3): 316-21.10. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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