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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig CD169 Antibody, clone 3B11/11    

Anti-Pig CD169 Antibody, clone 3B11/11 (FITC)

Mouse Anti-Pig Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A7LCJ3
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3B11/11
Calculated MW 184912 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Porcine alveolar macrophages.
Shelf Life 18 months from date of despatch.
Gene ID 397623
Other Names Sialoadhesin, pSn, Sialic acid-binding Ig-like lectin 1, Siglec-1, p210, SIGLEC1, SA, SN
Target/Specificity Mouse anti-Pig CD169, clone 3B11/11 Is specific for porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crockeret al.1986). CD169 has subsequently been identified in rat (van den Berg et al.1992), human (Mucklowet al.1995) and pig (Vanderheijdenet al.2003) . Mouse anti-Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti-porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullidoet al.1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputteet al.2006) and as a targeted receptor for the delivery of toxins and antigens (Delputteet al.2011) .Mouse anti-pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revillaet al).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig CD169 Antibody, clone 3B11/11 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens 49: 403-413. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet. Immunol. Immunopathol. 80: 93-109. 3. Revilla, C. et al. (2009) Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet. Res 40:1-15. 5. Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46.4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD12241
(40 western blots)
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