|Calculated MW||184912 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Sialoadhesin, pSn, Sialic acid-binding Ig-like lectin 1, Siglec-1, p210, SIGLEC1, SA, SN|
|Target/Specificity||Mouse anti-Pig CD169, clone 3B11/11 Is specific for porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crockeret al.1986). CD169 has subsequently been identified in rat (van den Berg et al.1992), human (Mucklowet al.1995) and pig (Vanderheijdenet al.2003) . Mouse anti-Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti-porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullidoet al.1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputteet al.2006) and as a targeted receptor for the delivery of toxins and antigens (Delputteet al.2011) .Mouse anti-pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revillaet al).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD169 Antibody, clone 3B11/11 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.Tissue Antigens 49: 403-413. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.Vet. Immunol. Immunopathol. 80: 93-109. 3. Revilla, C. et al. (2009) Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.Vet. Res 40:1-15. 5. Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.J Virol. 87: 9538-46.4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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