|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine alveolar macrophages.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig Macrophages antibody, clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.Some expression has also been observed on peripheral blood lymphocytes.The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer’s patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig Macrophages Antibody, clone BA4D5 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.Eur J Cell Biol. 87: 91-9. 2. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.Dev Comp Immunol. 33: 284-98. 3. Fujita M et al. (2013) Technique of endoscopic biopsy of islet allografts transplanted into the gastric submucosal space in pigs.Cell Transplant. 22 (12): 2335-44. 4. Liu, G. et al. (2015) Influenza A Virus Panhandle Structure is Directly Involved in RIG-I Activation and IFN Induction.J Virol. pii: JVI.00232-15. 5. Muscari C et al. (2010) Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage.Biol Proced Online. 12 (1): 9023. 6. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.Dev Comp Immunol. 33 (3): 284-98.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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