|Application ||WB, IHC-F, FC, IP|
|Calculated MW||90472 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA|
|Shelf Life||18 months from date of despatch.|
|Other Names||EGF-like module-containing mucin-like hormone receptor-like 2, EGF-like module receptor 2, CD312, EMR2|
|Target/Specificity||Mouse anti-Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Clone 2A1 specifically recognizes the stalk region of EMR2.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD312 Antibody, clone 2A1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kwakkenbos. M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.J. Leukoc. Biol.71:854-862 2. Stacey. M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chrondroitin sulphate glycosaminoglycans.Blood.102:2916-2924 3. Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.FASEB J. 22:741-51 4. Lin, H. et al. (2004) Autocatalytic cleavage of the EMR2 receptor occurs at a conserved G protein-coupled receptor proteolytic site motif.J Biol Chem. 279:31823-321. Kwakkenbos. M.J. et al. (2004) The EGF-TM7 family: a postgenomic view.Immunogenetics. 55:655-666
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