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>   home   >   Products   >   Primary Antibodies   >   Anti-Mouse Gr-1 Antibody, clone RB6-8C5 (RPE)   

Anti-Mouse Gr-1 Antibody, clone RB6-8C5

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse Gr-1 Antibody, clone RB6-8C5  ABD12255
    Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.Image caption:Depletion of the circulating monocyte and Gr1lo and F4/80hi populations following 1 week GW2580 treatment. A. Identification of total monocyte population in mouse blood using MAFIA-GFP. B Following one week of GW2580 treatment, no difference in total circulating monocytes was observed. FACS quantification of Gr1hi and Gr1lo (M2; C,D) and F4/80hi (E,F) in total monocytes following 1 week GW2580 treatment (n = 6 and n = 4 animals per group, respectively).From: Leblond A-L, Klinkert K, Martin K, Turner EC, Kumar AH, Browne T, et al. Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.PLoS ONE 10: e0137515.
  • IF - Anti-Mouse Gr-1 Antibody, clone RB6-8C5  ABD12255
    Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.Image caption:S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-μm-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A–C) S. Typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink . (A) Collapsed image from a 40-μm Z-stack. Scale bar is 20 μm. (B and C) Sections from (A) that are 4 µm apart. The video from which (A–C) were derived (Video S2) is available online. (D–G) T cells within multinucleate macrophages. Macrophages (F4–80 and MOMA-2) are blue (D, G, and H), T cells are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 μm. (H) An image from a different mouse stained and labeled as described for (D–G). Scale bar is 8 μm. A video showing a T cell inside of a macrophage is available online (Video S3).From: Nix RN, Altschuler SE, Henson PM, Detweiler CS Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3: e193.
  • FC - Anti-Mouse Gr-1 Antibody, clone RB6-8C5  ABD12255
    Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.Image caption:Effect of CD206+ M2 macrophage depletion on collagen deposition and cell infiltration within the infarct 2 weeks post MI in MAFIA mice. A. Representative Sirius Red staining on histological sections from MAFIA mice 2 weeks post MI. B. Quantification of collagen staining as a percentage of LV from MAFIA mice 2 weeks post MI (Scale bar: 1 mm, n = 6 animals per group, =8 images per animal). C. Representative hematoxilin and eosin staining on 20x infarct or remote histological section from MAFIA mice 2 weeks post MI showing an increase in inflammatory infiltrates in animals treated with GW2580. D. Quantification of nuclei number per mm2 in MAFIA remote and infarct zone in MAFIA mice 2 weeks post MI. (n = 4–6 animals per group). E. Representative images of immunofluorescent staining of CD206+ M2 macrophages, Gr1+ M1 macrophages and Ly6G+ neutrophils within the infarct zone from MAFIA mice 2 weeks post MI. Quantification of Ly6G+ neutrophil, Gr1+ M1 macrophage and CD206+ M2 macrophage infiltration within the infarct zone from MAFIA mice 2 weeks post MI (n = 4 animals per group, 10 images/animal). * p
  • FC - Anti-Mouse Gr-1 Antibody, clone RB6-8C5  ABD12255
    Staining of mouse peripheral blood granulocytes with Rat anti mouse Gr-1:Biotin
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35461
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names RB6-8C5
Calculated MW 11798 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Normal murine bone marrow cells.
Shelf Life 18 months from date of despatch.
Other Names Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g
Target/Specificity Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a 21–25kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Leeet al.2013). Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleminget al.1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendraet al.2007)The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo(Czuprynski, C.J. et al1994,Daleyet al.2008).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse Gr-1 Antibody, clone RB6-8C5 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
J Immunol. 151 (5): 2399-408. 2. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.
J Immunol. 147 (1): 22-8. 3. Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenesinfection.
J Immunol. 152 (4): 1836-46. 4. Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.
J Immunol. 185 (11): 7057-66. 5. Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.
J Pharmacol Exp Ther. 336: 381-90. 6. Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.
Blood.117: 1734-44. 7. Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Gué>rin immunotherapy of bladder cancer and orchestrate local immune responses.
Cancer Res. 66: 8250-7. 8. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
PLoS Pathog. 3: e193. 9. Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.
Endocrinology. 152: 3155-64. 10. Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.
J Exp Med. 179:259-68. 11. Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.
J Surg Res. 187 (2): 559-70. 12. Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
J Histochem Cytochem. 59: 813-25. 13. Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.
J Immunol.190: 340-8. 14. Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
Infect Immun. 82 (1): 253-64. 15. Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.
Am J Pathol. 185 (6): 1686-98. 16. Lee, Y.S. et al. (2015) Interleukin-1 signaling in intestinal stromal cells controls KC/CXCR1 secretion, which correlates with the recruitment of IL-22-secreting neutrophils at early stages of Citrobacter rodentiuminfection.
Infect Immun. pii: IAI.00670-15. 17. Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.
Eur J Immunol. 32 (11): 3235-45.

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Cat# ABD12255
(40 western blots)
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