|Application ||WB, IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Normal murine bone marrow cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g|
|Target/Specificity||Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a 21–25kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Leeet al.2013). Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleminget al.1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendraet al.2007)The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.Rat anti-Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo(Czuprynski, C.J. et al1994,Daleyet al.2008).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Gr-1 Antibody, clone RB6-8C5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.J Immunol. 151 (5): 2399-408. 2. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.J Immunol. 147 (1): 22-8. 3. Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenesinfection.J Immunol. 152 (4): 1836-46. 4. Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.J Immunol. 185 (11): 7057-66. 5. Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.J Pharmacol Exp Ther. 336: 381-90. 6. Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.Blood.117: 1734-44. 7. Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Gué>rin immunotherapy of bladder cancer and orchestrate local immune responses.Cancer Res. 66: 8250-7. 8. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.PLoS Pathog. 3: e193. 9. Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.Endocrinology. 152: 3155-64. 10. Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.J Exp Med. 179:259-68. 11. Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.J Surg Res. 187 (2): 559-70. 12. Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.J Histochem Cytochem. 59: 813-25. 13. Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.J Immunol.190: 340-8. 14. Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.Infect Immun. 82 (1): 253-64. 15. Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.Am J Pathol. 185 (6): 1686-98. 16. Lee, Y.S. et al. (2015) Interleukin-1 signaling in intestinal stromal cells controls KC/CXCR1 secretion, which correlates with the recruitment of IL-22-secreting neutrophils at early stages of Citrobacter rodentiuminfection.Infect Immun. pii: IAI.00670-15. 17. Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.Eur J Immunol. 32 (11): 3235-45.
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