|Application ||IHC-P, IHC-F, IF, FC|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Balb/c macrophage precursor cell hybrids.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Mouse CD301 antibody, clone ER-MP23 recognizes murine CD301, a 38 kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR).In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.Clone ER-MP23 is reported to block the function of mouse CD301. The antibody binds both MGL1 and MGL2 homologues.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD301 Antibody, clone ER-MP23 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Leenen, P.J.M. et. al. (1994) Markers of mouse macrophage development detected by monoclonal antibodies.J Immunol Methods. 174:5 – 19. 2. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.J Leukoc Biol. 78 (4): 845-52. 3. Abadie, V. et al. (2005) Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes.Blood. 106: 1843-50. 4. Dupasquier, M. et al. (2004) Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis.J Invest Dermatol. 123: 876-9. 5. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.J Clin Invest. 121: 985-97. 6. Westcott, D.J. et al. (2009) MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.J Exp Med. 206: 3143-56. 7. Fischer-Posovszky, P. et al. (2011) Targeted deletion of adipocytes by apoptosis leads to adipose tissue recruitment of alternatively activated m2 macrophages.Endocrinology. 152: 3074-81. 8. Spite, M. et al. (2011) Deficiency of the Leukotriene B4 Receptor, BLT-1, Protects against Systemic Insulin Resistance in Diet-Induced Obesity.J Immunol. 187: 1942-9. 9. Raes, G. et al. (2005) Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation.J Leukoc Biol. 77: 321-7. 10. Freire, T. et al. (2010) Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.Blood. 116: 3526-36. 11. Lumeng, C.N. et al. (2008) Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes.Diabetes. 57: 3239-46. 12. Blyszczuk, P. et al. (2013) Nitric oxide synthase 2 is required for conversion of pro-fibrogenic inflammatory CD133(+) progenitors into F4/80(+) macrophages in experimental autoimmune myocarditis.Cardiovasc Res. 97 (2): 219-29. 13. Dib, L.H. et al. (2014) Bone marrow leptin signaling mediates obesity-associated adipose tissue inflammation in male mice.Endocrinology. 155: 40-6. 14. Ferret-Bernard, S. et al. (2012) Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling.J. Proteomics. 75: 938-48. 15. Orr, J.S. et al. (2012) Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages.Diabetes. 61: 2718-27. 16. Wagner, M. et al. (2012) Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis.Angiogenesis.15: 481-95 17. Shah, R. et al. (2015) Metabolic Effects of CX3CR1 Deficiency in Diet-Induced Obese Mice.PLoS One. 10 (9): e0138317. 18. Morris, M.E. et al. (2015) Systemically Delivered Adipose Stromal Vascular Fraction Cells Disseminate to Peripheral Artery Walls and Reduce Vasomotor Tone Through a CD11b+ Cell-Dependent Mechanism.Stem Cells Transl Med. pii: sctm.2014-0252. 19. Vukman KV et al. (2013) Mannose receptor and macrophage galactose-type lectin are involved in Bordetella pertussis mast cell interaction.J Leukoc Biol. 94 (3): 439-48. 20. Hartwig, H. et al. (2015) Atherosclerotic Plaque Destabilization in Mice: A Comparative Study.PLoS One. 10 (10): e0141019.
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