|Application ||IHC-P, IHC-F, IF, FC|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Balb/c macrophage precursor cell hybrids.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Mouse CD301 antibody, clone ER-MP23 recognizes murine CD301, a 38 kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR).In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.Clone ER-MP23 is reported to block the function of mouse CD301. The antibody binds both MGL1 and MGL2 homologues.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD301 Antibody, clone ER-MP23 is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Leenen, P.J.M. et. al. (1994) Markers of mouse macrophage development detected by monoclonal antibodies.J Immunol Methods. 174:5 – 19. 2. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.J Leukoc Biol. 78 (4): 845-52. 3. Abadie, V. et al. (2005) Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes.Blood. 106: 1843-50. 4. Dupasquier, M. et al. (2004) Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis.J Invest Dermatol. 123: 876-9. 5. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.J Clin Invest. 121: 985-97. 6. Westcott, D.J. et al. (2009) MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.J Exp Med. 206: 3143-56. 7. Fischer-Posovszky, P. et al. (2011) Targeted deletion of adipocytes by apoptosis leads to adipose tissue recruitment of alternatively activated m2 macrophages.Endocrinology. 152: 3074-81. 8. Spite, M. et al. (2011) Deficiency of the Leukotriene B4 Receptor, BLT-1, Protects against Systemic Insulin Resistance in Diet-Induced Obesity.J Immunol. 187: 1942-9. 9. Raes, G. et al. (2005) Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation.J Leukoc Biol. 77: 321-7. 10. Freire, T. et al. (2010) Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.Blood. 116: 3526-36. 11. Lumeng, C.N. et al. (2008) Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes.Diabetes. 57: 3239-46. 12. Blyszczuk, P. et al. (2013) Nitric oxide synthase 2 is required for conversion of pro-fibrogenic inflammatory CD133(+) progenitors into F4/80(+) macrophages in experimental autoimmune myocarditis.Cardiovasc Res. 97 (2): 219-29. 13. Dib, L.H. et al. (2014) Bone marrow leptin signaling mediates obesity-associated adipose tissue inflammation in male mice.Endocrinology. 155: 40-6. 14. Ferret-Bernard, S. et al. (2012) Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling.J. Proteomics. 75: 938-48. 15. Orr, J.S. et al. (2012) Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages.Diabetes. 61: 2718-27. 16. Wagner, M. et al. (2012) Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis.Angiogenesis.15: 481-95 17. Shah, R. et al. (2015) Metabolic Effects of CX3CR1 Deficiency in Diet-Induced Obese Mice.PLoS One. 10 (9): e0138317. 18. Morris, M.E. et al. (2015) Systemically Delivered Adipose Stromal Vascular Fraction Cells Disseminate to Peripheral Artery Walls and Reduce Vasomotor Tone Through a CD11b+ Cell-Dependent Mechanism.Stem Cells Transl Med. pii: sctm.2014-0252. 19. Vukman KV et al. (2013) Mannose receptor and macrophage galactose-type lectin are involved in Bordetella pertussis mast cell interaction.J Leukoc Biol. 94 (3): 439-48. 20. Hartwig, H. et al. (2015) Atherosclerotic Plaque Destabilization in Mice: A Comparative Study.PLoS One. 10 (10): e0141019.
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