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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD178 Antibody, clone 10F2    

Anti-Human CD178 Antibody, clone 10F2

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD178 Antibody, clone 10F2  ABD12258
    Staining of CD178 transfected CHO cells with Mouse anti Human CD178
  • FC - Anti-Human CD178 Antibody, clone 10F2  ABD12258
    Staining of CD178 transfected CHO cells with Mouse anti Human CD178
  • E - Anti-Human CD178 Antibody, clone 10F2  ABD12258
    Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma sample is displayed at 1:2 dilution.
  • FC - Anti-Human CD178 Antibody, clone 10F2  ABD12258
    Staining of CD178 transfected CHO cells with Mouse anti Human CD178
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48023
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 10F2
Calculated MW 31485 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Gene ID 356
Other Names Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178, Tumor necrosis factor ligand superfamily member 6, membrane form, Tumor necrosis factor ligand superfamily member 6, soluble form, Receptor-binding FasL ectodomain, Soluble Fas ligand, sFasL, ADAM10-processed FasL form, APL, FasL intracellular domain, FasL ICD, SPPL2A-processed FasL form, SPA, FASLG, APT1LG1, CD95L, FASL, TNFSF6
Target/Specificity Mouse anti-Human CD178 antibody, clone 10F2 recognizes the human CD178 cell surface antigen, a ~40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis. Clone 10F2 is reported to block the activity of Fas ligand (Legembreet al.2002).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD178 Antibody, clone 10F2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Legembre, P. et al. (2002) Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas.
Cell Death Differ. 9 (3): 329-39. 2. Ferry-Dumazet, H. et al. (2002) Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells.
Carcinogenesis. 23 (8): 1327-33. 3. Thorén, F.B. et al. (2012) Human NK Cells induce neutrophil apoptosis via an NKp46- and Fas-dependent mechanism.
J Immunol. 188 (4): 1668-74. 4. Robinet, P. et al. (2014) A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
J Immunol. 192 (11): 5332-42. 5. Pellegrin, S. et al. (2012) Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by epo-withdrawal.
PLoS One. 7 (6): e38356.

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$ 514.00
$ 144.00
Cat# ABD12258
2 Formats Available
Availability: 5-7days
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