|Application ||IHC-F, FC, IP|
|Other Accession||P16109, P42201|
|Calculated MW||83517 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||P-selectin transfected 300.19 cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P, Selp|
|Target/Specificity||Mouse anti-human CD62P, clone Psel.KO.2.7, recognizes human P-Selectin. CD62P is a 140kDa surface antigen expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD62P Antibody, clone Psel.KO.2.7 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).Tissue antigens 56: 117-128. 2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).Vet. Immunol. Immunopathol. 96: 169-181. 3. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-343. 4. Shirasuna, K. et al. (2012) Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.PLoS One. 7: e29054. 5. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.Artif Organs. 35: 602-13. 6. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).Vet Immunol Immunopathol. 164 (1-2): 87-92. 7. Johnson, C.A. Jr et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.Artif Organs. 32 (2): 136-45. 8. Johnson, C.A. Jr et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.Artif Organs. 35 (1): 9-21. 9. Johnson, C.A. Jr et al. (2011) Platelet activation after implantation of the Levitronix PediVAS in the ovine model.ASAIO J. 57 (6): 516-21. 10. Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.ASAIO J. 60 (4): 429-35. 11. Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.BMC Vet Res. 9: 144.
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