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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Bovine CD25 Antibody, clone IL-A111 (RPE)   

Anti-Bovine CD25 Antibody, clone IL-A111 (FITC)

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti-Bovine CD25 Antibody, clone IL-A111 (FITC) ABD12268
    Published customer image:Mouse anti Bovine CD25 antibody used to evaluate CD25 expression on NKp46 expressing cells following in vitro culture by flow cytometry.Image caption: Phenotype of cultured NKp46+ populations. Ovine NKp46+ populations after 7-8 days of in vitro culture were stained with monoclonal antibodies against CD2, CD8, CD16 and CD25 (solid lines) or isotype controls (broken lines). Results are shown from one culture that was representative of those derived from five animals.From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW. NKp46 defines ovine cells that have characteristics corresponding to NK cells.Vet Res. 2011 Feb 23;42:37.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12342
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names IL-A111
Calculated MW 31239 Da
Additional Information
Other Species Sh
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Shelf Life 18 months from date of despatch.
Gene ID 281861
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
Target/Specificity Mouse anti-Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.Clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessenset al.1992).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Bovine CD25 Antibody, clone IL-A111 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Naessens, J. et al. (1992) Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene.
Immunology 76: 305-309. 2. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2).
Vet Immunol. Immunopathol. 39:25-48. . 3. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J. Immunol. 159: 5372-7382. 4. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet. Immunol. Immunopathol. 39: 283-290. 5. Evans, C.W. et al. (1994) Antigen recognition and activation of ovine gamma delta T cells.
Immunology. 82: 229-237. 6. Campbell, J.D.M. et al. (1998) A novel cell surface proliferation- associated marker expressed on T cells and up-regulated on germinal center B cells.
J. Leukoc. Biol. 63: 567-574. 7. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. Feb 42: 37. 8. Menge, C. et al. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection.
J Med Microbiol. 53: 573-9. 9. Rhodes, S.G. et al. (1999) Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.
J Gen Virol. 80 : 1673-9. 10. Piper, E.K. et al. (2009) Immunological profiles of Bos taurus and Bos indicus cattle infested with the cattle tick, Rhipicephalus (Boophilus) microplus.
Clin Vaccine Immunol. 16: 1074-86. 11. Woolums, A.R. et al. (2013) Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
Am J Vet Res. 74: 343-54. 12. Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
Immunology. 96: 396-403. 13. Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
Pol J Vet Sci. 15: 11-20. 14. Menge, C. et al. (2003) Verotoxin 1 from Escherichia coli affects Gb3/CD77+ bovine lymphocytes independent of interleukin-2, tumor necrosis factor-alpha, and interferon-alpha.
Exp Biol Med (Maywood). 228: 377-86. 15. Menge, C. et al. (1999) Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
Infect Immun. 67: 2209-17. 16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75. 17. Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
Pol J Vet Sci. 15 (1): 11-20. 18. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. 19. Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. 20. Kang, H.J. et al. (2016) Effects of Ambient Temperature on Growth Performance, Blood Metabolites, and Immune Cell Populations in Korean Cattle Steers.
Asian-Australas J Anim Sci. 29 (3): 436-43.

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Cat# ABD12268
(40 western blots)
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