|Application ||FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Bovine peripheral blood monouclear cells.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Bovine CD45RO, clone IL-A116, specifically recognises the bovine homologue of the human CD45RO cell surface antigen.CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. Studies utilizing clone IL-A116 have demonstrated that the percentage of CD45RO+CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogget al.2011). It has been demonstrated that mouse anti-bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of 180kDa (Bembridgeet al1995) which is analogus to the molecular weight of human and mouse CD45RO.Clone IL-A116 has been demonstrated to recognise the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus(Bembridgeet al1995).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Bovine CD45RO Antibody, clone IL-A116 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bembridge, G.P.et al. (1995) CD45RO expression on bovine T cells: relation to biological function.Immunology 86: 537-544. 2. Hogg A.E. et. al. (2011) Characterization of age-related changes in bovine CD8+ T-cellsVet. Immunol. Immunopath. 140:47-54 3. Whelan A.O. et. al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine TuberculosisPLoS ONE 6(12): e29194. doi:10.1371/journal.pone.0029194 4. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-48. 5. Sopp, P. et al. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR+ T cells from cattle lymph nodes and blood.Vet. Immunol. Immunopathol. 81: 85-96. 6. McInnes, E.et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.Immunology 96: 396-403 7. Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).Vet. Immunol. Immunopathol. 39:129-136. 8. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet. Immunol. Immunopathol. 39: 283-290.
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