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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (RPE)   

Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC)

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC) ABD12271
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic)
  • FC - Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC) ABD12271
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II :FITC
  • FC - Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC) ABD12271
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II :RPE
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names IL-A21
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howardet al.1997).Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells. Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Collen, T. et al. (1991) A T cell epitope in VP1 of Foot-and-mouth disease virus is immunodominant for vaccinated cattle.
J. Immunol. 146: 749-755. 2. Davies, S.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
Eur. J. Immunogenet. 19: 253-262. 3. DeMartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
Vet. Immunol. Immunopathol. 35: 253-273. 4. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J. Immunol. 159: 5372-7382. 5. Ballingall, K.T. et al. (2001) Transcription of the unique ruminant class II major histocompatability complex- DYAandDIB genes in dendritic cells.
Eur. J. Immunol. 31: 82-86. 6. Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
Immunology. 105:181-9 7. Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
Infect Immun. 67:2241-9. 8. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75.

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Cat# ABD12271
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