Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC)
Mouse Anti-Bovine Monoclonal Antibody
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ (Howardet al.1997).Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells. Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Bovine MHC Class II Monomorphic Antibody, clone IL-A21 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Collen, T. et al. (1991) A T cell epitope in VP1 of Foot-and-mouth disease virus is immunodominant for vaccinated cattle.J. Immunol. 146: 749-755. 2. Davies, S.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.Eur. J. Immunogenet. 19: 253-262. 3. DeMartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.Vet. Immunol. Immunopathol. 35: 253-273. 4. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.J. Immunol. 159: 5372-7382. 5. Ballingall, K.T. et al. (2001) Transcription of the unique ruminant class II major histocompatability complex- DYAandDIB genes in dendritic cells.Eur. J. Immunol. 31: 82-86. 6. Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.Immunology. 105:181-9 7. Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parvaInfect Immun. 67:2241-9. 8. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.Int J Parasitol. 40: 865-75.
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