|Application ||IHC-F, FC|
|Calculated MW||53864 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant human C3aR protein|
|Shelf Life||18 months from date of despatch.|
|Other Names||C3a anaphylatoxin chemotactic receptor, C3AR, C3a-R, C3AR1, AZ3B, C3R1, HNFAG09|
|Target/Specificity||Mouse anti-Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a 55kD cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes. Expression levels of C3aR on human cells varies significantly between donors.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human C3a Receptor Antibody, clone hC3aRZ1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Werfel, T. et.al. (2000) Activated human T lymphocytes express a functional C3a receptor.J Immunol. 165:6599 – 6605. 2. Soruri, A. et.al (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.J Immunol.170:3306 - 3314. 3. Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.Immunology. 97: 166-72. 4. Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.J Immunol. 166: 3410-5. 5. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.J Immunol. 183: 506-17. 6. Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.PLoS One. 6: e18812. 7. Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.PLoS One. 10: e0116772. 8. Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.J Am Soc Nephrol. 20: 289-98. 9. Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.Immunol Lett. 143: 131-6. 10. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.Atherosclerosis. 238 (2): 289-95.
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