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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Glucocorticoid Receptor Antibody, clone 5E4 (FITC)   

Anti-Human Glucocorticoid Receptor Antibody, clone 5E4

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04150
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 5E4
Calculated MW 85659 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography
Immunogen 26 amino acid peptide corresponding to residues 150-176 on human GCR linked to thyroglobulin.
Shelf Life 18 months from date of despatch.
Gene ID 2908
Other Names Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Target/Specificity Mouse anti-Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins (Kinoet al.2009), or the nucleus following binding to ligand (Werbet al.1978).Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels (Malchoffet al.1993).Mouse anti-human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor (Bergquistet al.2014)
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Glucocorticoid Receptor Antibody, clone 5E4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Berki, T. et al. (1998) Production and flow cytometric application of a monoclonal anti-glucocorticoid receptor antibody.
J. Immunol. Methods 214: 19-27. 2. Kim, S.Y. et al. (2010) Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line
Korean J Pediatr 53: 753-8 3. Bergquist, M. et al. (2014) Glucocorticoid receptor function is decreased in neutrophils during endotoxic shock.
J Infect. pii: S0163-4453(14)00081-4.

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Cat# ABD12280
(40 western blots)
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