|Application ||IHC-F, IF, FC, IP, Func|
|Calculated MW||33516 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||B lymphoblastoid cell line HFB1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74, CD74, DHLAG|
|Target/Specificity||Mouse anti-Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD74 Antibody, clone Bu45 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Wraight, C.J. et al. (1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface. J Biol Chem.265:5787 – 5792. 2. Beswick, E.J. et al. (2005) Helicobacter pylori binds to CD74 on gastric epithelial cells and stimulates interleukin-8 production.Infect Immun. 73: 2736-43. 3. Lapaque, N. et al. (2009) The HLA-DRalpha chain is modified by polyubiquitination.J Biol Chem. 284: 7007-16. 4. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP.Proc Natl Acad Sci U S A. 94: 7499-502. 5. Barrera, C.A. et al. (2005) Polarized expression of CD74 by gastric epithelial cells.J Histochem Cytochem. 53: 1481-9. 6. Beswick, E.J. et al. (2006) The Helicobacter pylori urease B subunit binds to CD74 on gastric epithelial cells and induces NF-kappaB activation and interleukin-8 production.Infect Immun. 74: 1148-55.
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