|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6|
|Target/Specificity||Mouse anti-Human CD47 antibody, clone 1/1A4 recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1. Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells (Waclaviceket al.1993).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD47 Antibody, clone 1/1A4 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Waclavicek, M. et al. (1997) T cell stimulation via CD47: Agonistic and antagonistic effects of CD47 monoclonal antibody 1/1A4. J. Immunol. 159: 5345-5354.1. Frazier, W.A. et al. (1999) The thrombospondin receptor integrin-associated protein (CD47) functionally couples to heterotrimeric Gi*.J. Biol. Chem. 274: 8554-8560.
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