|Application ||IHC-F, FC, IP|
|Calculated MW||182624 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human Rhinovirus (HRV14) treated monocyte-derived dendritic cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, SIGLEC1, SN|
|Target/Specificity||Mouse anti-Human CD169 antibody, clone 7-239 specifically recognizes human CD169, otherwise known as Sialoadhesin (Sn), a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1. CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis. Clone 7-239 completely inhibits erythrocyte rosetting around Sn+ transductants.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD169 Antibody, clone 7-239 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kirchberger, S. et al. (2005) Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression.J Immunol. 175 (2): 1145-52. 2. Izquierdo-Useros, N. et al. (2012) Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides.PLoS Biol. 10: e1001448. 3. Rose, T. et al. (2013) IFNa and its response proteins, IP-10 and SIGLEC-1, are biomarkers of disease activity in systemic lupus erythematosus.Ann Rheum Dis. 72 (10): 1639-45. 4. Akiyama H et al. (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies.PLoS Pathog. 11 (3): e1004751.1. Hartnell, A. et al. (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.Blood. 97 (1): 288-96.
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