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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD16 Antibody, clone DJ130c (RPE)   

Anti-Human CD16 Antibody, clone DJ130c

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD16 Antibody, clone DJ130c  ABD12300
    Published customer image:Characterization of human monocyte susets using Mouse anti Human CD16 antibody, clone DJ130c by flow cytometry.Image caption:Monocyte subsets and expression of phenotypic markers on spondyloarthritis (SpA) and rheumatoid arthritis (RA) patients and healthy donor monocytes. The percentage of CD14+CD16-, CD14+CD16+, and CD14dimCD16+ monocytes from SpA and RA patients and healthy donors was measured with flow cytometry (A). Expression of CD64, CD200R, and CD16 on monocytes from SpA and RA patients and healthy donors was measured with flow cytometry and expressed as geometric mean fluorescence intensity (gMFI). Data are represented as median and interquartile range of at least seven independent experiments (B). From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL. Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis. Arthritis Res Ther. 2012 Apr 11;14:R74.
  • FC - Anti-Human CD16 Antibody, clone DJ130c  ABD12300
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD16
  • FC - Anti-Human CD16 Antibody, clone DJ130c  ABD12300
    Published customer image:Mouse anti Human CD16 antibody, clone DJ130c used for the detection of CD16 on in vitropolarized human macrophages by flow cytometry.Image caption:Expression of phenotypic markers on in vitro polarized human macrophages. Healthy peripheral blood monocytes were cultured for 4 days in medium or in medium supplemented with IFN-?, IL-4, IL-10, or LPS in the absence (white bars) or presence (black bars) of HAGGs. Expression of MFIFN-? markers CD80 and CD64, MFIL-4 markers CD200R and CD14, and MFIL-10 markers CD163, CD16 and CD32 was measured by flow cytometry. Bars represent the mean ± SEM of 4 independent experiments. *p
  • FC - Anti-Human CD16 Antibody, clone DJ130c  ABD12300
    Published customer image:Mouse anti Human CD16 antibody, clone DJ130c for the evaluation of CD16 expression on monocytes by flow cytometry.Image caption:Identification of monocyte-platelet aggregates by flow cytometry. A, Gating strategy using forward and side scatter to select monocytes, side scatter vs CD14 expression to exclude granulocytes and ungated CD14 vs CD16 expression to exclude natural killer lymphocytes. Subsets were defined as CD14++CD16–CCR2+ , CD14++CD16+CCR2+ , and CD14+CD16++CCR2- monocytes. B, Monocyte-platelet aggregates defined as monocytes expressing the platelet surface marker CD42a.From: Wrigley BJ, Shantsila E, Tapp LD, Lip GY. Increased formation of monocyte-platelet aggregates in ischemic heart failure. Circ Heart Fail. 2013 Jan;6(1):127-35.
  • FC - Anti-Human CD16 Antibody, clone DJ130c  ABD12300
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD16:Alexa Fluor® 647
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC
Primary Accession O75015
Other Accession P08637
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DJ130c
Calculated MW 26216 Da
Additional Information
Other Species Macaque
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Gene ID 2215
Other Names Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3
Target/Specificity Mouse anti-Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallonet al.1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype.Mouse anti-Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta(Xuet al.2012)
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD16 Antibody, clone DJ130c is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Schmidt, R.E. (1993) CD16 cluster workshop report.
In Leucocyte Typing V: White cell differentiation antigens, Vol.1. Edited by Schlossman, S.F. et al. Oxford University Press. p805 – 806. 3. Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. 4. Shantsila, E. et al. (2012) Fibrinolytic status in acute coronary syndromes: evidence of differences in relation to clinical features and pathophysiological pathways.
Thromb Haemost. 108: 32-40. 5. Shantsila, E. et al. (2011) Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
J Thromb Haemost. 9: 1056-66. 6. Tapp, L.D. et al. (2012) The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction.
J Thromb Haemost. 10: 1231-41. 7. Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 14: R74. 8. Ambarus, C.A. et al. (2012) Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
J Immunol Methods. 375: 196-206. 9. Ambarus, C.A. et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
PLoS One. 7: e35994. 10. Shantsila, E. et al. (2012) The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates.
Eur J Clin Invest. 42: 832-9. 11. Chehadeh. W. et al. (2009) Antibody-mediated opsonization of red blood cells in parvovirus B19 infection.
Virology. 390: 56-63. 12. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6: 127-35. 13. Jaipersad, A.S. et al. (2014) Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis.
Ann Med. 11: 1-9. 14. Shantsila, E. et al. (2015) Free Light Chains in patients with acute coronary syndromes: Relationships to inflammation and renal function.
Int J Cardiol. 185: 322-7. 15. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6 (1): 127-35. 16. Romee R et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17).
Blood. 121 (18): 3599-608.

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