|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine bone marrow haematopoietic cells (BMHC).|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig Granulocytes antibody, clone 6D10 recognizes a 60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (product code (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.Clone 6D10 has been shown as suitable for use on cytospins (Perezet al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig Granulocytes Antibody, clone 6D10 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Perez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers. Dev. Comp. Immunol. 31: 296-306. 2. Stone JP et al. (2015) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.Am J Transplant. Sep 14. [Epub ahead of print] 3. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.Dev Comp Immunol. 33 (3): 284-98.2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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