|Application ||IHC-F, FC, IP|
|Calculated MW||32780 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Mouse CD274 - transfected L5178Y cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1|
|Target/Specificity||Rat anti-Mouse CD274 antibody, clone MIH6 detects mouse CD274, also known as B7-H1 and PD-1L, a single pass type I cell membrane glycoprotein, a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells (Ishadaet al.2002), endothelial cells (Eppihimeret al.2002) and epithelial cells in response to Interferons alpha, beta and gamma.CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells (Selenko-Gebaueret al.2003). CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10 (Caoet al.2003). CD274 has also been shown to costimulate early T cell priming and differentiation.Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers (Iwaiet al.2002).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD274 Antibody, clone MIH6 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.J Immunol. 171 (8): 4156-63. 2. Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.J Immunol. 169 (10): 5538-45. 3. Furuhashi, K. et al. (2011) Mouse Lung CD103+and CD11bhigh dendritic cells preferentially induce distinct CD4+ T cell responses.Am J Respir Crit Care Med 181: 2010: A3795 4. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012: 172420. 5. Haile, S.T. et al. (2013) Soluble CD80 Restores T Cell Activation and Overcomes Tumor Cell Programmed Death Ligand 1-Mediated Immune Suppression.J Immunol. 191: 2829-36. 6. Lopez-Medina, M. et al.(2015)Salmonella induces PD-L1 expression in B cells.Immunol Lett. pii: S0165-2478(15)30018-3. 7. Yao, L. et al. (2016) Characterization of Liver Monocytic Myeloid-Derived Suppressor Cells and Their Role in a Murine Model of Non-Alcoholic Fatty Liver Disease.PLoS One. 11 (2): e0149948.
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