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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine CD14 Antibody, clone CC-G33    

Anti-Bovine CD14 Antibody, clone CC-G33 (FITC)

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti-Bovine CD14 Antibody, clone CC-G33 (FITC) ABD12322
    Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD14
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q95122
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC-G33
Calculated MW 39667 Da
Additional Information
Other Species Sh,H,Water Buffalo
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.
Shelf Life 18 months from date of despatch.
Gene ID 281048
Other Names Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, CD14
Target/Specificity Mouse anti-Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Mouse anti-Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Soppet al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielliet al.2013.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Bovine CD14 Antibody, clone CC-G33 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Sopp, P. et al. (1996) Identification of bovine CD14.
Vet Immunol Immunopathol. 52 (4): 323-8. 2. Villarreal-Ramos, B. et al. (2006) Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle.
Vaccine. 24 (47-48): 6850-8. 3. Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.
Vet Immunol Immunopathol. 52 (4): 245-8. 4. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80. 5. Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
Immunology. 105: 190-5. 6. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
J Leukoc Biol. 83: 1396-403. 7. Herath, S. et al. (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus.
Endocrinology. 147: 562-70. 8. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 11: 262-72. 9. Villarreal-Ramos, B. et al. (2003) Investigation of the role of CD8+ T cells in bovine tuberculosis in vivo.
Infect Immun.71: 4297-303. 10. Leung, S.T. et al. (2000) Uterine lymphocyte distribution and interleukin expression during early pregnancy in cows.
J Reprod Fertil. 119: 25-33. 11. Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 43: 54. 12. Gibson, A. et al. (2012) Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
Dev Comp Immunol. 36: 332-41. 13. Haas, K.M. and Estes, D.M. (2001) The identification and characterization of a ligand for bovine CD5.
J Immunol. 166: 3158-66. 14. Miarelli, M. et al. (2013) Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance
Open J Anim Sci. 3 (2): 127-31. 15. Altreuther, G. et al. (2001) Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.
Scand J Immunol. 54: 459-69. 16. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. 17. Vrieling, M. et al. (2015) Bovine Staphylococcus aureus Secretes the Leukocidin LukMF' To Kill Migrating Neutrophils through CCR1.
MBio. 6 (3): e00335.

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Cat# ABD12322
(40 western blots)
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