|Calculated MW||39667 Da|
|Other Species||Sh,H,Water Buffalo|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, CD14|
|Target/Specificity||Mouse anti-Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Mouse anti-Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Soppet al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielliet al.2013.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Bovine CD14 Antibody, clone CC-G33 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Sopp, P. et al. (1996) Identification of bovine CD14.Vet Immunol Immunopathol. 52 (4): 323-8. 2. Villarreal-Ramos, B. et al. (2006) Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle.Vaccine. 24 (47-48): 6850-8. 3. Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.Vet Immunol Immunopathol. 52 (4): 245-8. 4. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.J Gen Virol. 84: 1771-80. 5. Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.Immunology. 105: 190-5. 6. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.J Leukoc Biol. 83: 1396-403. 7. Herath, S. et al. (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus.Endocrinology. 147: 562-70. 8. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.Immunology. 11: 262-72. 9. Villarreal-Ramos, B. et al. (2003) Investigation of the role of CD8+ T cells in bovine tuberculosis in vivo.Infect Immun.71: 4297-303. 10. Leung, S.T. et al. (2000) Uterine lymphocyte distribution and interleukin expression during early pregnancy in cows.J Reprod Fertil. 119: 25-33. 11. Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.Vet Res. 43: 54. 12. Gibson, A. et al. (2012) Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon responseDev Comp Immunol. 36: 332-41. 13. Haas, K.M. and Estes, D.M. (2001) The identification and characterization of a ligand for bovine CD5.J Immunol. 166: 3158-66. 14. Miarelli, M. et al. (2013) Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistanceOpen J Anim Sci. 3 (2): 127-31. 15. Altreuther, G. et al. (2001) Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.Scand J Immunol. 54: 459-69. 16. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.Theriogenology. 82 (3): 403-10. 17. Vrieling, M. et al. (2015) Bovine Staphylococcus aureus Secretes the Leukocidin LukMF' To Kill Migrating Neutrophils through CCR1.MBio. 6 (3): e00335.
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