|Purification||Purified IgG prepared by affinity chromatography from tissue culture supernatant|
|Immunogen||Rat thymocyte membrane glycoproteins|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-MHC Class II H-2I-Ab/s antibody, clone OX-3 recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Mouse anti-MHC Class II H-2I-Ab/s antibody, clone OX-3 is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.Mouse anti-MHC Class II H-2I-Ab/s antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.This product is routinely tested in flow cytometry on Lewis rat splenocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-MHC Class II H-2I-Ab/s Antibody, clone OX-3 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.Eur. J. Immunol. 9: 426-433. 2. McMaster, W. R. and Williams, A. F. (1979) Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins.Immunol. Rev. 47: 117-137. 3. Barclay, A.N. and Mayrhofer G. (1981) Bone marrow origin of Ia-positive cells in the medulla of rat thymus.J. Exp. Med. 153: 1666-1671. 4. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42: 593-600. 5. Zhang, J. et al. (1997) Expression of major histocompatibility complex molecules in rodent retina.Immunohistochemical study. Invest. Ophthalmol. Vis. Sci. 38 (9): 1848 - 1857. 6. Hahm. K. et al. (2000) Loss of TGF - beta signaling contributes to autoimmune pancreatitis.J. Clin. Invest. 105: 1057 - 1065.
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