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>   home   >   Products   >   Primary Antibodies   >   Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (RPE)   

Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC)

Mouse Anti-Rat Monoclonal Antibody

     
  • FC - Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) ABD12325
    Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing mictoglial cells in rat brain by immunohistochemistry on gormalin fixed cryosections.Image caption:Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic disease. A, low power view of midbrain reveals enhanced lectin staining in the red nucleus. B, higher magnification shows that enhanced lectin reactivity is confined strictly to red nucleus region (arrows indicate perimeter of red nucleus). C, microglial fusions are interspersed with rubrospinal neurons that appear undamaged. D, lectin-positive microglial fusion (giant cell) within red nucleus. E, oculomotor nucleus reveals normal-appearing motor neurons and lack of microgliosis. F, substantia nigra (pars compacta) shows presence of normal, ramified microglial cells. G, motor cortex shows normal, ramified microglia. H, single, ramified microglial cell positive with OX-6 (arrow) near lateral ventricle. Scale bars: 400 μm (A); 200 μm (E); 100 μm (B,H); 50 μm (C,F,G); 20 μm (D).From: Fendrick SE, Xue QS, Streit WJ. Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene.J Neuroinflammation. 2007 Feb 28;4:9.
  • IHC-P - Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) ABD12325
    Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing cells in the rat brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Representative images of histochemical staining of brain tissue (TBI and control) with ED-1, OX-6, GFAP, Perl's, and Fluoro-Jade B ten days after the microdialysis experiment.From: Folkersma H, Foster Dingley JC, van Berckel BN, Rozemuller A, Boellaard R, Huisman MC, Lammertsma AA, Vandertop WP, Molthoff CF. Increased cerebral (R)-[C]PK11195 uptake and glutamate release in a rat model of traumatic brain injury: a longitudinal pilot study. J Neuroinflammation. 2011 Jun 14;8:67.
  • IHC-P - Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) ABD12325
    Puiblished customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used to identify activated microglia in rat brain be immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Imatinib reduces infiltration of immune cells and attenuates microglia activation. IHC analysis on paraffin embedded spinal cord tissue cross-sections on day 10 p.i. (A–D; n = 8 rats/experimental group; representative images shown) and 14 p.i. (E–H; n = 5 rats/experimental group; representative images shown). Although showing no sign of CNS inflammation and demyelination, control animals already started recruiting W3/13+ T-cells (B) and ED1+ macrophages (D) to the meninges and in the perivascular space, whereas the imatinib-treated group showed a delay in recruitment of inflammatory cells to the CNS. On day 14 p.i., spinal cords of the imatinib-treated rats exhibiting demyelinated lesions recruited lower amounts of W3/13+ T-cells (G, H) while ED1+ macrophages infiltration was similar to the controls (E, F). Scale bar, 200 μm (A–H). (I–Z) IF performed on spinal cord cross-sections of rats injected with fluorescent tracer (dextran, red) on day 14 p.i. α-Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 antibody staining (all in green) in imatinib- (I, L, O, R, U, X) and PBS-treated rats (J, M, P, S, V, Y). (I–K) Microglia activation was significantly decreased in the imatinib-treated rats, while Ox-42+ cells were detectable around leaky blood vessels in the control tissue. (L–N) The amount of macrophages/activated microglia cells was significantly decreased in the spinal cords of the imatinib-treated rats. (O–Q) Significantly lower amounts of MHC class II+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls. (R–Z) Significantly lower amounts of Ox-22+, CD45RA+ and W3/13+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls (R, U, X vs. S, V, Y). Quantifications of Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 expression based on green fluorescent pixel area quantifications from spinal cord cross-sections (K, N, Q, T, W, Z). n = 5 rats/experimental group. Scale bar, 50 μm. Error bars, S.E.M. Statistics were calculated using t-test and P values <0.05 were considered significant (P<0.05 = *, P<0.01 = **, P<0.001 = ***). Imatinib or PBS oral gavage was performed from day 5 p.i until the end of the experiment.From: Z. Adzemovic M, Zeitelhofer M, Eriksson U, Olsson T, Nilsson I Imatinib Ameliorates Neuroinflammation in a Rat Model of Multiple Sclerosis by Enhancing Blood-Brain Barrier Integrity and by Modulating the Peripheral Immune Response.PLoS ONE 8(2): e56586.
  • IHC - Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) ABD12325
    Published customer imageMouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections.Image caption:Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200 μm; higher magnification - 20 μm. Abbreviations: V, lateral ventricle; IC, infarct core.From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment.J Neuroinflammation. 2014 Aug 23;11:145.
  • IHC - Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) ABD12325
    Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the visualization of MHC Class II expressing cells in Rat brain by immunohistochemistry on formalin fixed coronal tissue sections.Image caption:Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 μm. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5–6/group, ** p<0.01, *** p
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names OX-6
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Rat thymocyte membrane glycoproteins.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-MHC Class II H-2I-Ak/s antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.Mouse anti-MHC Class II H-2I-Ak/s antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male, D. K. et al.).MCA2687A647 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster & Williams 1979) and (Maleet al.1987).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region. This product is routinely tested in flow cytometry on rat splenocytes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur. J. Immunol. 9: 426-433. 2. Fernandez, J. L. and Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.
Lab. Anim. 20: 293-297. 3. Charteris, D. G. and Lightman, S. L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78: 387-392. 4. Whiteland, J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J. Histochem. Cytochem. 43: 313-320. 5. McKechnie, N. M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.
J. Infect. Dis. 176: 1334-1343. 6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain
J. Immunogenet. 14: 301-312. 8. Meyer zu Hörste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules.
PLoS One. 6(6): e21223. 9. Zhang, M. et al. (2015) The distinct distributions of immunocompetent cells in rat dentin pulp after pulpotomy.
Anat Rec (Hoboken). 298 (4): 741-9.

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