|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Rat thymocyte membrane glycoproteins.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-MHC Class II H-2I-Ak/s antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.Mouse anti-MHC Class II H-2I-Ak/s antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male, D. K. et al.).MCA2687A647 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster & Williams 1979) and (Maleet al.1987).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region. This product is routinely tested in flow cytometry on rat splenocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-MHC Class II H-2I-Ak/s Antibody, clone OX-6 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.Eur. J. Immunol. 9: 426-433. 2. Fernandez, J. L. and Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens.Lab. Anim. 20: 293-297. 3. Charteris, D. G. and Lightman, S. L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.Immunology. 78: 387-392. 4. Whiteland, J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.J. Histochem. Cytochem. 43: 313-320. 5. McKechnie, N. M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia.J. Infect. Dis. 176: 1334-1343. 6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strainJ. Immunogenet. 14: 301-312. 8. Meyer zu Hörste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules.PLoS One. 6(6): e21223. 9. Zhang, M. et al. (2015) The distinct distributions of immunocompetent cells in rat dentin pulp after pulpotomy.Anat Rec (Hoboken). 298 (4): 741-9.
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