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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD3 Antibody, clone 145-2C11 (FITC)   

Anti-Mouse CD3 Antibody, clone 145-2C11

Hamster Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD3 Antibody, clone 145-2C11  ABD12326
    Mouse splenocytes stained with Hamster anti Mouse CD3:AlexaFluor®488
  • IF - Anti-Mouse CD3 Antibody, clone 145-2C11  ABD12326
    Published customer image:Hamster anti Mouse CD3 antibody, clone 145-2C11 used for the identification of murine colonic lymphocytes by immunofluorescence.Image caption:Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.PLoS Med 5: e239.
  • FC - Anti-Mouse CD3 Antibody, clone 145-2C11  ABD12326
    Mouse splenocytes stained with Hamster anti Mouse CD3
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22646
Reactivity Mouse
Host Hamster
Clonality Monoclonal
Isotype IgG
Clone Names 145-2C11
Calculated MW 21393 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Shelf Life 18 months from date of despatch.
Gene ID 12501
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, Cd3e
Target/Specificity Hamster anti-Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.Hamster anti-Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD3 Antibody, clone 145-2C11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
Proc Natl Acad Sci U S A. 84 (5): 1374-8. 2. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
J Immunol. 146 (8): 2536-43. 3. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
J Immunol. 153 (11): 5176-82. 4. Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.
J Immunol. 176 (11): 6532-42. 5. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33. 6. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
PLoS Med. 5: e239. 7. Klemann, C. et al. (2015) Interleukin-17, Produced by ?d-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.
Gastroenterology. pii: S0016-5085(15)01352-9.

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Cat# ABD12326
(40 western blots)
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