|Application ||WB, IHC-F, IF, FC, IP|
|Calculated MW||21393 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, Cd3e|
|Target/Specificity||Hamster anti-Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.Hamster anti-Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD3 Antibody, clone 145-2C11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 84 (5): 1374-8. 2. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.J Immunol. 146 (8): 2536-43. 3. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.J Immunol. 153 (11): 5176-82. 4. Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.J Immunol. 176 (11): 6532-42. 5. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.Am J Pathol. 177 (4): 1823-33. 6. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.PLoS Med. 5: e239. 7. Klemann, C. et al. (2015) Interleukin-17, Produced by ?d-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.Gastroenterology. pii: S0016-5085(15)01352-9.
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