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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse CD8 alpha Antibody, clone 53-6.7 (RPE-CY7)   

Anti-Mouse CD8 alpha Antibody, clone 53-6.7

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD8 alpha Antibody, clone 53-6.7  ABD12329
    Figure A. Alexa® 647 conjugated mouse anti human CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa® 647 conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD8 alpha . All experiments performed on red cell lysed murine splenocytes gated on lymphocytes in the presence of murine SeroBlock
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01731
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 53-6.7
Calculated MW 27456 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Mouse thymus or spleen.
Shelf Life 18 months from date of despatch.
Gene ID 12525
Other Names T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2
Target/Specificity Rat anti-Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. Rat anti-Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD8 alpha Antibody, clone 53-6.7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Jabbari, A. and Harty, J.T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses.
J. Leukoc. Biol. 80: 16-23. 2. Takahashi, K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells.
Proc Natl Acad Sci U S A. 89 (12): 5557-61. 2. McDole, J.R. et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33.

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Cat# ABD12329
(40 western blots)
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