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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human CD144 Antibody    

Anti-Human CD144 Antibody

Rabbit Anti-Human Polyclonal Antibody

  • IF - Anti-Human CD144 Antibody  ABD12332
    Published customer image:Rabbit anti Human CD144 antibody used for the detection of CD144 (ve-cadherin) expressing cells in vitroImage caption:Immunofluorescence staining of VE-cadherin (A,B) and von Willebrand factor (C,D) in CPAE. Cells cultivated on (Alb/Hep)2 (A,C) and on (Alb/Hep)2 with the adsorbed FGF-2ads (30 ng/cm2; (Alb/Hep)2FGF-2adsLow; B,D). The cells are counterstained with Hoechst 33342. Obj. ×20 (A,B), obj. ×10 (C,D), scale bar = 200 µm.From: Kumorek M, Kubies D, Filová E, Houska M, Kasoju N, Mázl Chánová E, et al. Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies.PLoS ONE 10: e0125484.
  • IF - Anti-Human CD144 Antibody  ABD12332
    Published customer image:Effects of fibronectin and collagen on the immunophenotype of ECFCs. ECFCs expanded on fibronectin or collagen showed a similar immunophenotype. On the left are reported flow cytometric histograms showing that, independently from the substrate used for cell culture, ECFCs did not express CD45 and CD14, while they expressed at similar levels CD31, VEGFR2, CD144, CD146, CD54 and CXCR4. On the right are reported immunofluorescence photographs showing that ECFCs expanded on both substrates incorporated Dil-ac-LDL and bound lectin UEA-1. Photographs were obtained using an an Olympus Fluoview FV1000 confocal microscope, x40 magnification. One of three representative experiments is reported.From: Colombo E, Calcaterra F, Cappelletti M, Mavilio D, Della Bella S Comparison of Fibronectin and Collagen in Supporting the Isolation and Expansion of Endothelial Progenitor Cells from Human Adult Peripheral Blood. PLoS ONE 8(6): e66734.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, FC, E, Func
Primary Accession P33151
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 87528 Da
Additional Information
Other Species B,M,Rat
Purification Antisera to human CD144 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin.
Shelf Life 18 months from date of despatch.
Gene ID 1003
Other Names Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144, CDH5
Target/Specificity Rabbit anti-Human CD144 antibody recognizes human CD144, also known as VE-cadherin. CD144 is a ~130 KDa cell surface glycoprotein expressed by endothelial cells. Rabbit anti-Human CD144 antibody does not cross react with N-cadherin, which is also expressed by endothelial cells. Rabbit anti-Human CD144 antibody is reported to block adhesive properties of VE-cadherin (Gulino, D. et al.1998).
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Human CD144 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Gulino, D. et al. (1998) Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin.
J. Biol. Chem. 273: 29786 - 29793. 2. Koga, H. (2005) Elevated levels of VE-cadherin-positive endothelial microparticles in patients with type 2 diabetes mellitus and coronary artery disease.
J Am Coll Cardiol. 45:1622-30. 3. Rigolin, G.M. et al. (2010) Circulating endothelial cells in patients with chronic lymphocytic leukemia: clinical-prognostic and biologic significance.
Cancer. 116:1926-37 4. Machalinska, A. et al. (2011) Different populations of circulating endothelial cells in patients with age-related macular degeneration: a novel insight into pathogenesis.
Invest Ophthalmol Vis Sci. 52 (1): 93-100. 5. Kuczmarski, J.M. et al. (2011) Effect of moderate-to-severe chronic kidney disease on flow-mediated dilation and progenitor cells.
Exp Biol Med (Maywood). 236 (9): 1085-92. 6. Glading, A. et al. (2007) KRIT-1/CCM1 is a Rap1 effector that regulates endothelial cell cell junctions.
J Cell Biol. 179: 247-54. 7. Kumorek, M. et al. (2015) Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies.
PLoS One. 10 (5): e0125484.

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Cat# ABD12332
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