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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD40 Antibody, clone 3/23 (RPE)   

Anti-Mouse CD40 Antibody, clone 3/23

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD40 Antibody, clone 3/23  ABD12336
    Staining of mouse splenocytes with Rat anti Mouse CD40: Low Endotoxin
  • FC - Anti-Mouse CD40 Antibody, clone 3/23  ABD12336
    Staining of mouse splenocytes with Rat anti Mouse CD40: Alexa Fluor® 647
  • FC - Anti-Mouse CD40 Antibody, clone 3/23  ABD12336
    Staining of mouse splenocytes cells with Rat anti Mouse CD40
  • FC - Anti-Mouse CD40 Antibody, clone 3/23  ABD12336
    Staining of mouse splenocytes with Rat anti Mouse CD40:RPE
  • FC - Anti-Mouse CD40 Antibody, clone 3/23  ABD12336
    Staining of mouse splenocytes with Rat anti Mouse CD40:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27512
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 3/23
Calculated MW 32093 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Shelf Life 12 months from date of reconstitution.
Gene ID 21939
Other Names Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40, Cd40, Tnfrsf5
Target/Specificity Rat anti-Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti-Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.Rat anti-Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD40 Antibody, clone 3/23 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Parry, S.L. et al. (1994) Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes.
Eur J Immunol. 24 (4): 974-9. 2. Hasbold, J. et al. (1994) Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies.
Eur J Immunol. 24 (8): 1835-42. 3. Hasbold, J. & Klaus, G.G. (1994) B cells from CBA/N mice do not proliferate following ligation of CD40.
Eur J Immunol. 24 (1): 152-7. 4. Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
J Clin Invest. 119 (12): 3723-38. 5. Mohan, J. et al. (2005) Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure.
Immunology. 116: 122-33. 6. Mukhopadhyay, S. et al. (2004) Activation of murine macrophages by Neisseria meningitidis and IFN-gamma in vitro: distinct roles of class A scavenger and Toll-like pattern recognition receptors in selective modulation of surface phenotype.
J Leukoc Biol. 76: 577-84. 7. Russo, S. et al. (2003) Platelet-activating factor mediates CD40-dependent angiogenesis and endothelial-smooth muscle cell interaction.
J Immunol. 171: 5489-97. 8. Shakib, S. et al. (2009) Checkpoints in the development of thymic cortical epithelial cells.
J Immunol. 182: 130-7. 9. Ogasawara, K. et al. (2002) Profiles of cell-to-cell interaction of Mycobacterium intracellulare-induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission.
Clin Exp Immunol. 129: 272-80. 10. Salomon, B. et al. (1998) Three populations of mouse lymph node dendritic cells with different origins and dynamics.
J Immunol. 160: 708-17. 11. Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
Int Immunol. 12: 1669-75. 12. Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
J Immunol. 187: 4517-29. 13. Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
Eur J Immunol. 45 (2): 480-91.

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Cat# ABD12336
(40 western blots)
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