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>   home   >   Products   >   Primary Antibodies   >   Anti Pig CD45 Antibody, clone K252.1E4    

Anti-Pig CD45 Antibody, clone K252.1E4

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig CD45 Antibody, clone K252.1E4  ABD12338
    Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD45:Alexa Fluor®647
  • FC - Anti-Pig CD45 Antibody, clone K252.1E4  ABD12338
    Staining of pig peripheral blood lymphocytes with Mouse anti Pig CD45:FITC
  • FC - Anti-Pig CD45 Antibody, clone K252.1E4  ABD12338
    Published customer image:Mouse anti Pig CD45 antibody, clone K252.1E4 used for the identification of contaminating leukocytes in bone marrow derived mesenchymal sem cell cultures by flow cytomery.Image caption:Characterization of pig BM-MSCs. (A) Single-cell suspension plated at 4000 cells/cm2 formed abundant colony forming units (CFUs, purple dots) after 9 days of culturing. The average number of CFUs (>50 cells/CFU) was 158.8±7.0. (B–D) Upon proper induction, osteogenic (B), chondrogenic (C) and adipogenic (D) differentiations of pig BM-MSC were confirmed by their characteristic stains, respectively . Calibration bars: 50 μm. (E–F) Flow cytometric analysis of obtained pig BM-MSCs in the first three passages showed positive expression of MSC surface markers (CD44 and CD105; E) and negative expression of hematopoietic surface markers (CD45 and CD11b; F).From: Sun Z, Tee BC, Kennedy KS, Kennedy PM, Kim D-G, Mallery SR, et al. Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model.PLoS ONE 8(9): e74672.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names K252.1E4
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A.
Immunogen Porcine Peripheral Blood lymphocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig CD45, clone K252.1E4 recognizes an epitope common to all porcine CD45 isoforms (Schnitzleinet al.1998). CD45 is also known as leukocyte common antigen (LCA).Mouse anti-Pig CD45, clone K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190 kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermannet al.1994).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Pig CD45 Antibody, clone K252.1E4 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis Sci. 43 (5): 1488-92. 2. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
Vet Res Commun. 26: 273-83. 3. Barker, E. et al. (2006) The larynx as an immunological organ: immunological architecture in the pig as a large animal model.
Clin Exp Immunol. 143: 6-14. 4. Vilahur, G. et al. (2015) Roflumilast-induced Local Vascular Injury Is Associated with a Coordinated Proteome and Microparticle Change in the Systemic Circulation in Pigs.
Toxicol Pathol. 43 (4): 569-80. 5. O'Leary, S. et al. (2004) Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig.
Reproduction. 128: 237-47. 6. Zelnickova, P. et al. (2006) Postnatal functional maturation of blood phagocytes in pig.
Vet Immunol Immunopathol. 113: 383-91. 7. Bimczok, D. et al. (2006) Phenotype and distribution of dendritic cells in the porcine small intestinal and tracheal mucosa and their spatial relationship to epithelial cells.
Cell Tissue Res. 325: 461-8. 8. Nochi, T. et al. (2004) Biological role of Ep-CAM in the physical interaction between epithelial cells and lymphocytes in intestinal epithelium.
Clin Immunol. 113: 326-39. 9. Bimczok, D. et al. (2010) Primary porcine CD11R1+ antigen-presenting cells isolated from small intestinal mucosa mature but lose their T cell stimulatory function in response to cholera toxin treatment.
Vet Immunol Immunopathol. 134: 239-48. 10. Ebdrup, L. et al. (2008) Dynamic expression of the signal regulatory protein alpha and CD18 on porcine PBMC during acute endotoxaemia.
Scand J Immunol. 68: 430-7. 11. Plánka, L. et al. (2009) Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs.
Physiol Res. 58: 885-93. 12. Plánka, L. et al. (2009) Comparison of Preventive and Therapeutic Transplantations of Allogeneic Mesenchymal Stem Cells in Healing of the Distal Femoral Growth Plate Cartilage Defects in Miniature Pigs.
Acta Vet. Brno 78: 293-302. 13. Plánka, L. et al. (2008) New options for management of posttraumatic articular cartilage defects.
Rozhl Chir. 87: 42-5. 14. Splíchal, I. et al. (2002) Escherichia coli Administered into Pig Amniotic Cavity Appear in Fetal Airways and Attract Macrophages into Fetal Lungs.
Physiol Res. 51: 523-8. 15. Juhásová, J. et al. (2011) Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment.
Physiol Res. 60: 559-71. 16. Heino, T.J. et al. (2012) Comparison of the osteogenic capacity of minipig and human bone marrow-derived mesenchymal stem cells.
J Orthop Res. 30: 1019-25. 17. Stepanova, H. et al. (2012) CD4+ and γδTCR+ T lymphocytes are sources of interleukin-17 in swine.
Cytokine. 58: 152-7. 18. Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-ß-D-glucan supplementation in neonatal piglets.
Clin Vaccine Immunol. 19: 1499-508. 19. Noort, W.A. et al. (2011) Human versus porcine mesenchymal stromal cells: phenotype, differentiation potential, immunomodulation and cardiac improvement after transplantation.
J Cell Mol Med. 16: 1827-39. 20. Thorum, S.C. et al. (2013) Impact of diet on development of bronchial-associated immunity in the neonatal piglet.
Vet Immunol Immunopathol. 151: 63-72. 21. Tran, L.A. et al. (2010) Gadonanotubes as Magnetic Nanolabels for Stem Cell Detection
Biomaterials. 31: 9482-91. 22. Thorum, S.C. et al. (2013) Dietary (1,3/1,6)-ß-D-glucan decreases transforming growth factor ß expression in the lung of the neonatal piglet.
Nutr Res. 33: 322-31. 23. Koskinas, K.C. et al. (2013) Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs.
Int J Cardiol. 169 (6): 394-401. 24. Franzoni, G. et al. (2013) Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge.
Clin Vaccine Immunol. 20: 1604-16. 25. Filová E, et al. (2009) Regionally-selective cell colonization of micropatterned surfaces prepared by plasma polymerization of acrylic acid and 1,7-octadiene.
Physiol Res. 58: 669-84. 26. Bimczok, D. et al. (2005) Site-specific expression of CD11b and SIRPalpha (CD172a) on dendritic cells: implications for their migration patterns in the gut immune system.
Eur J Immunol. 35: 1418-27. 27. Sun, Z. et al. (2013) Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model.
PLoS One. 8: e74672. 28. Post, I.C. et al. (2013) Characterization and quantification of porcine circulating endothelial cells.
Xenotransplantation. 20: 18-26. 29. Foubert P et al. (2015) Uncultured adipose-derived regenerative cells (ADRCs) seeded in collagen scaffold improves dermal regeneration, enhancing early vascularization and structural organization following thermal burns.
Burns. pii: S0305-4179(15)00132-1. 1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD12338
(40 western blots)
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