|Calculated MW||115696 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||P815 DBA/2 murine mastocytoma cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, Lymphocyte Peyer patch adhesion molecules subunit alpha, LPAM subunit alpha, VLA-4 subunit alpha, CD49d, Itga4|
|Target/Specificity||Rat anti-Mouse CD49d monoclonal antibody, clone PS/2 recognizes murine alpha 4 integrin (CD49d), a ~150kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmannet al.1989). CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1 (Sheppardet al.1994).Clone PS/2 has also been reported to block the binding of CD49d to its ligands (Andrewet al.1994).|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD49d Antibody, clone PS/2 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Miyake, K. et al. (1991) Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis.J. Exp. Med. 173: 599-607. 2. Miyake, K. et al. (1991) A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture.J. Cell Biol. 114: 557-565. 3. Andrew, D.P. et al. (1994) Distinct but Overlapping Epitopes Are Involved in alpha4 beta7-Mediated Adhesion to Vascular Cell Adhesion Molecule-1, Mucosal Addressin-1, Fibronectin, and Lymphocyte Aggregation.J. Immunol. 153:3847 - 3861. 4. Bellingan, G.J. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.J Exp Med. 196: 1515-21. 5. Fukuoka, M. et al. (2000) Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines.Blood. 96: 4267-75. 6. Hokibara, S. et al. (2000) Effects of monoclonal antibodies to adhesion molecules on eosinophilic myocarditis in Toxocara canis-infected CBA/J mice.Clin Exp Immunol. 114: 236-44. 7. Li, W. et al. (2008) Reduced alpha4beta1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase deficient mice.Glycobiology. 18: 114-24. 8. Liu, Z.J. et al. (1999) A novel role for H-Ras in the regulation of very late antigen-4 integrin and VCAM-1 via c-Myc-dependent and -independent mechanisms.J Immunol. 163: 4901-8. 9. Tchilian, E.Z. et al. (1997) Anti-alpha 4 integrin antibody induces apoptosis in murine thymocytes and staphylococcal enterotoxin B-activated lymph node T cells.Immunology. 92: 321-7. 10. Bowden, R.A. et al. (2002) Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium.Circ Res. 90: 562-9. 11. Hirata, T. et al. (2002) P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin.J Immunol. 169: 4307-13. 12. Vaz, R. et al. (2012) Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.Cell Tissue Res. 348: 569-78. 13. Gillberg, L. et al. (2013) Effective treatment of mouse experimental colitis by alpha 2 integrin antibody: comparison with alpha 4 antibody and conventional therapy.Acta Physiol (Oxf). 207: 326-36. 14. Eshghi, S. et al. (2007) Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: integrins in red cell development.J Cell Biol. 177: 871-80. 15. Maus, U.A. et al. (2004) Pneumolysin-induced lung injury is independent of leukocyte trafficking into the alveolar space.J Immunol. 173: 1307-12. 16. Enghofer, M. et al. (1998) Lymphocyte transfer in streptozotocin-induced diabetes: adhesion of donor cells to islet endothelium.Am J Physiol. 274: E928-35. 17. Zhang, Y. et al. (2012) Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells.J Immunol. 189: 3914-24. 18. Ferrer, P. et al. (2005) Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma.Neoplasia. 7: 37-47. 19. Tanneau, G.M. et al. (1999) Differential recruitment of T- and IgA B-lymphocytes in the developing mammary gland in relation to homing receptors and vascular addressins.J Histochem Cytochem. 47: 1581-92. 20. Omenetti, S. et al. (2015) Dysregulated intrahepatic CD4+ T-cell activation drives liver inflammation in ileitis-prone SAMP1/YitFc mice.Cell Mol Gastroenterol Hepatol. 1 (4): 406-419.
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