|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine CD45RB, clone CC76, recognizes the RB isoform of the bovine Leucocyte Common Antigen expressed in subpopulations of CD4+ and CD8+ T-cells. Expression levels of CD45RB are used as a marker differentiating naïve and memory T lymphocytes (Howard, C. et al., 1991).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine CD45RB Antibody, clone CC76 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Howard, C.J. et al. (1991) Distinction of naive and memory Bo CD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte common antigen, CD45.Eur. J. Immunol. 21: 2219-2226. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle.Vet. Immunol. Immunopathol. 39: 25-48. 3. Sopp, P. and Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8, and WC1 gamma-delta TCR+ cells from cattle lymph nodes and blood. Vet. Immunol.Immunopathol. 81: 85-96. 4. Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).Vet. Immunol. Immunopathol. 39: 129 - 136. 5. Reid, E. et al. (2011) Bovine plasmacytoid dendritic cells are the major source of type I interferon in response to foot-and-mouth disease virus in vitro and in vivo.J Virol. 85: 4297-308.
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