|Application ||IHC-F, FC|
|Calculated MW||33077 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Other Names||B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20|
|Target/Specificity||Mouse anti-Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti-Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyaket al.2002).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD20 Antibody, clone 2H7 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Hultin, L.E. et al. (1993) CD20 (pan B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes.Cytometry 14: 196. 2. Lifson, J. et al. (2001) Role of CD8+ lymphocytes in control of simian Immunodefiency virus infection and resistance to rechallenge after transient early antiretroviral treatment. J. Virol. 75 (21): 10187 - 10199. 3. Jaramillo, M.C. et al. (2009) Increased manganese superoxide dismutase expression or treatment with manganese porphyrin potentiates dexamethasone-induced apoptosis in lymphoma cells.Cancer Res. 69: 5450-7. 4. Teeling, J.L. et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked tounique epitopes on CD20.J. Immunol. 177: 362-71. 5. Chan, H.T. et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts.Cancer Res. 63: 5480-9. 6. Cragg, M.S. et al. (2003) Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts.Blood. 101: 1045-52. 7. van den Akker, E. et al. (2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.Haematologica. 95: 1594-8. 8. Polyak, M.J. et al. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.Blood. 1;99:3256-62. 9. Greig, B. et al. (2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.Cytometry B Clin Cytom. 86: 135-8. 10. Jaramillo, M.C. et al. (2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.Free Radic Biol Med. 83: 89-100. 11. Kohler SL et al. (2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.J Immunol. Feb 12. pii: 1502174. [Epub ahead of print] .
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