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Anti-Human Actin (Muscle) Antibody, clone HHF35

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68032
Other Accession P62736, P63267, P68133
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names HHF35
Calculated MW 42019 Da
Additional Information
Other Species M,Cat,Rb,Japanese Macaque,Dog,Rat,C,Macaque,Shrew
Immunogen Muscle actin, purified from human myocardium
Shelf Life 18 months from date of despatch.
Gene ID 70
Other Names Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC
Target/Specificity Mouse anti-Human actin (muscle) antibody, clone HHF35, recognizes muscle specific actin, a 42kDa protein specific for actin present in skeletal, cardiac and smooth muscle. Mouse anti-Human actin (muscle) antibody, (HHF35) recognizes alpha actin and does not recognize beta actin.Mouse anti-Human actin (muscle) antibody, clone HHF35 may be useful in the study of tumors such as rhabdomyosarcomas, leiomyomas and leiomyosarcomas, arising from muscle cells.Mouse anti-Human actin (muscle) antibody, clone HHF35 is reported to be suitable for use in Western blotting (Tsukadaet al.1987).
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Actin (Muscle) Antibody, clone HHF35 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Tsukada, T. et al. (1987) HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.
Am. J. Pathol. 126: 51 2. Wu, M.H. et al. (2011) Targeting Galectin-1 in Carcinoma-Associated Fibroblasts Inhibits Oral Squamous Cell Carcinoma Metastasis by Downregulating MCP-1/CCL2 Expression.
Clin Cancer Res. 17: 1306-16. 3. Perez, J. et al. (1996) Immunohistochemical characterization of hemangiopericytomas and other spindle cell tumors in the dog.
Vet. Pathol. 33 (4): 391-397. 4. Hirata, Y. et al. (1994) Experimental intimal thickening studies using the photochemically induced thrombosis model in the guinea pig femoral artery.
Atherosclerosis 107(1): 117-124. 5. Martin de las Mulas, J. et al. (1994) Immunohistochemical distribution pattern of intermediate filament proteins and muscle actin in feline and human mammary carcinomas.
J. Comp. Pathol. 111(4): 365-81. 6. Shiomi, M. et al. (1994) Cell compositions of coronary and aortic atherosclerotic lesions in WHHL rabbits differ. An immunohistochemical study.
Arterioscler Thromb. 14(6): 931-937. 7. Tsubura, A. et al. (1991) Immunophenotypic difference of Keratin expression in normal mammary glandular cells from five different species.
Acta Anat. 140: 287 - 293. 8. Burgess, K.L. et al. (1996) Myoepithelial cells actively proliferate during atrophy of rat parotid gland.
Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 82: 674 - 680. 9. Verberne, M. et al. (2000) Distribution of antigen epitopes shared by nerves and the myocardium of the embryonic chick heart using different neuronal markers.
The Anatomical Record. 260: 335 - 350.

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Cat# ABD12345
(40 western blots)
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