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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse CD153 Antibody, clone RM153 (RPE)   

Anti-Mouse CD153 Antibody, clone RM153 (Biotin)

Rat Anti-Mouse Monoclonal Antibody

     
  • FC - Anti-Mouse CD153 Antibody, clone RM153 (Biotin) ABD12348
    Staining of Con A and PMA activated EL4 cells with Rat anti Mouse CD153: Alexa Fluor® 488
  • FC - Anti-Mouse CD153 Antibody, clone RM153 (Biotin) ABD12348
    Staining of Con A activated mouse spleen cells with Rat anti Mouse CD153: FITC
  • FC - Anti-Mouse CD153 Antibody, clone RM153 (Biotin) ABD12348
    Staining of Con A activated mouse spleen with Rat anti Mouse CD153: FITC
  • FC - Anti-Mouse CD153 Antibody, clone RM153 (Biotin) ABD12348
    Staining of Con A and PMA activated EL4 cells with Rat anti Mouse CD153: Alexa Fluor® 647
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P32972
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names RM153
Calculated MW 26519 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Mouse CD153/CHO cells.
Shelf Life 18 months from date of despatch.
Gene ID 21949
Other Names Tumor necrosis factor ligand superfamily member 8, CD30 ligand, CD30-L, CD153, Tnfsf8, Cd30l, Cd30lg
Target/Specificity Rat anti-Mouse CD153 antibody, clone RM153 recognizes murine CD153, also known as (CD30 ligand), a 40 kDa type II transmembrane protein belonging to the TNF superfamily.In the mouse CD153 is expressed primarily on activated CD4+ve T cells. The interaction of CD153 with its ligand CD30 is reported to enhance the proliferation of anti-CD3/CD28 co-stimulated T-cells.Clone RM153 is reported to block the functional binding of CD153 and can be used to inhibit the binding of murine CD30 to CD153 (Shimozatoet al.1999).
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD153 Antibody, clone RM153 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Shimozato, O. et al. (1999) Expression of CD30 ligand (CD153) on murine activated T cells.
Biochem Biophys Res Commun. 256 (3): 519-26. 2. Akiba, H. et al. (2000) Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis.
J Exp Med. 191: 375-80. 3. Mori, M. et al. (1999) CD30-CD30 ligand interaction in primary cutaneous CD30(+) T-cell lymphomas: A clue to the pathophysiology of clinical regression.
Blood. 94: 3077-83.

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Cat# ABD12348
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