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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10    

Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10  ABD12359
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 1 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)
  • IHC-P - Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10  ABD12359
    Published customer image:Mouse anti Human estrogen receptor beta 1, clone PPG5/10 used for the evaluation of ERβ1 expression in endometrial cancer tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue cestions.Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERα was low/absent in poor grade cancers (J) but immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.BMC Cancer. 2009 Sep 16;9:330.
  • IHC-P - Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10  ABD12359
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 1 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)
  • IHC-P - Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10  ABD12359
    Published customer image:Mouse anti Human estrogen receptor beta 1, clone PPG5/10 used for the demonstration of ERβ1 expression in ovine fetal liver and kidneys by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Role of estrogen. Circulating estradiol levels in control females (white bars) and males (black bars) and those exposed to TP quantified through RIA (A). Immunolocalisation of ERS1 in a representative liver (B) and kidney (C) in the d90 female fetus. Inset is negative control. Renal (light bars) and hepatic (dark bars) ERS1 expression in d90 females, whose mothers were prenatally treated with control oil or TP (D). Values represent mean ±S.E.M. ** P<0.01. Scale bars represent 100μm.From: Connolly F, Rae MT, Späth K, Boswell L, McNeilly AS, Duncan WC In an Ovine Model of Polycystic Ovary Syndrome Prenatal Androgens Suppress Female Fetal Renal Gluconeogenesis.PLoS ONE 10(7): e0132113.
  • IHC-P - Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10  ABD12359
    Staining of paraffin embedded human breast cancer tissue with Mouse anti Human estrogen receptor following antigen retrieval with EDTA. Detection was performed with Histar detection kit
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF
Primary Accession Q92731
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names PPG5/10
Calculated MW 59216 Da
Additional Information
Other Species Primate,New World primate,Llama,Rat,Marmoset,Pig,Sh,Cynomolgus Pr,Rh,Turtle,Goose
Immunogen Synthetic peptide sequence CSPAEDSKSKEGSQNPQSQ from the C-terminal region of the human estrogen receptor beta 1 isoform.
Shelf Life 18 months from date of despatch.
Gene ID 2100
Other Names Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
Target/Specificity Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow.Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (Shaaban, A.M.et al.), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts. Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 has also been reported to stain cells in the adenohypophysis, demonstrating cytoplasmic expression and is suitable for use in Western blotting (Shaaban, A.M.et al.)
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Moore, J.T. et al. (1998) Cloning and characterization of human estrogen receptor beta isoforms.
Biochem Biophys Res Commun. 247 (1): 75-8. 2. Torlakovic, E. et al. (2002) Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score.
Hum Pathol. 33 (6): 646-51. 3. Saunders, P.T. et al. (2002) Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.
Br J Cancer. 86 (2): 250-6. 4. Schiessl, B. et al. (2005) Expression of endothelial NO synthase, inducible NO synthase, and estrogen receptors alpha and beta in placental tissue of normal, preeclamptic, and intrauterine growth-restricted pregnancies.
J Histochem Cytochem. 53 (12): 1441-9. 5. Wong NA et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivoandin vitro study of clinicopathological and molecular correlates.
J Pathol. 207 (1): 53-60. 6. Fixemer, T. et al. (2003) Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma.
Prostate. 54: 79-87. 7. Wood, C.E. et al. (2006) Hyperplastic and neoplastic lesions of the mammary gland in macaques.
Vet Pathol. 43 (4): 471-83. 8. Juengel, J. L. et al. (2006) Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep.
Reproduction. 131: 81-92. 9. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
BMC Cancer. 9: 330. 10. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.
Eur J Endocrinol. 157 (2): 239-44. 11. Bianchi, C. et al. (2010) Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama).
Reprod Domest Anim. 45 (5): 872-80. 12. Marotti, J.D. et al. (2010) Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study.
Mod Pathol. 23: 197-204. 13. Saunders, P.T. et al. (2000) Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans.
Biol Reprod. 63 (4): 1098-105. 14. Asgari, M. and Morakabati, A. (2011) Estrogen receptor beta expression in prostate adenocarcinoma.
Diagn Pathol. 6: 61. 15. Fowler, P.A. et al. (2011) Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary.
J Clin Endocrinol Metab. 96: 1754-62. 16. Mafuvadze, B. et al. (2011) Apigenin Prevents Development of Medroxyprogesterone Acetate-Accelerated 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Sprague-Dawley Rats.
Cancer Prev Res (Phila). 4: 1316-24. 17. Silvestri, A. and Fraser, H.M. (2007) Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy.
Reproduction. 134: 341-53. 18. Bombail, V. et al. (2008) Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle.
Hum Reprod. 23: 2782-90. 19. Nagai H et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS One. 10 (7): e0131923. 20. Leska A et al. (2015) Estradiol concentration and the expression of estrogen receptors in the testes of the domestic goose (Anser anserf.domestica) during the annual reproductive cycle.
Domest Anim Endocrinol. 51: 96-104.

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