|Application ||IHC-P, IHC-F, IF|
|Calculated MW||59216 Da|
|Other Species||Primate,New World primate,Llama,Rat,Marmoset,Pig,Sh,Cynomolgus Pr,Rh,Turtle,Goose|
|Immunogen||Synthetic peptide sequence CSPAEDSKSKEGSQNPQSQ from the C-terminal region of the human estrogen receptor beta 1 isoform.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2|
|Target/Specificity||Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow.Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (Shaaban, A.M.et al.), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts. Mouse anti-Human estrogen receptor beta 1, clone PPG5/10 has also been reported to stain cells in the adenohypophysis, demonstrating cytoplasmic expression and is suitable for use in Western blotting (Shaaban, A.M.et al.)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Estrogen Receptor beta 1 Antibody, clone PPG5/10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Moore, J.T. et al. (1998) Cloning and characterization of human estrogen receptor beta isoforms.Biochem Biophys Res Commun. 247 (1): 75-8. 2. Torlakovic, E. et al. (2002) Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score.Hum Pathol. 33 (6): 646-51. 3. Saunders, P.T. et al. (2002) Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.Br J Cancer. 86 (2): 250-6. 4. Schiessl, B. et al. (2005) Expression of endothelial NO synthase, inducible NO synthase, and estrogen receptors alpha and beta in placental tissue of normal, preeclamptic, and intrauterine growth-restricted pregnancies.J Histochem Cytochem. 53 (12): 1441-9. 5. Wong NA et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivoandin vitro study of clinicopathological and molecular correlates.J Pathol. 207 (1): 53-60. 6. Fixemer, T. et al. (2003) Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma.Prostate. 54: 79-87. 7. Wood, C.E. et al. (2006) Hyperplastic and neoplastic lesions of the mammary gland in macaques.Vet Pathol. 43 (4): 471-83. 8. Juengel, J. L. et al. (2006) Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep.Reproduction. 131: 81-92. 9. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.BMC Cancer. 9: 330. 10. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.Eur J Endocrinol. 157 (2): 239-44. 11. Bianchi, C. et al. (2010) Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama).Reprod Domest Anim. 45 (5): 872-80. 12. Marotti, J.D. et al. (2010) Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study.Mod Pathol. 23: 197-204. 13. Saunders, P.T. et al. (2000) Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans.Biol Reprod. 63 (4): 1098-105. 14. Asgari, M. and Morakabati, A. (2011) Estrogen receptor beta expression in prostate adenocarcinoma.Diagn Pathol. 6: 61. 15. Fowler, P.A. et al. (2011) Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary.J Clin Endocrinol Metab. 96: 1754-62. 16. Mafuvadze, B. et al. (2011) Apigenin Prevents Development of Medroxyprogesterone Acetate-Accelerated 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Sprague-Dawley Rats.Cancer Prev Res (Phila). 4: 1316-24. 17. Silvestri, A. and Fraser, H.M. (2007) Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy.Reproduction. 134: 341-53. 18. Bombail, V. et al. (2008) Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle.Hum Reprod. 23: 2782-90. 19. Nagai H et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.PLoS One. 10 (7): e0131923. 20. Leska A et al. (2015) Estradiol concentration and the expression of estrogen receptors in the testes of the domestic goose (Anser anserf.domestica) during the annual reproductive cycle.Domest Anim Endocrinol. 51: 96-104.
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