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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human CD284 Antibody, clone HTA125 (RPE)   

Anti-Human CD284 Antibody, clone HTA125

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD284 Antibody, clone HTA125  ABD12360
    Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor® 488
  • FC - Anti-Human CD284 Antibody, clone HTA125  ABD12360
    Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor® 647
  • FC - Anti-Human CD284 Antibody, clone HTA125  ABD12360
    Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC
  • FC - Anti-Human CD284 Antibody, clone HTA125  ABD12360
    Staining of human peripheral blood monocytes with Mouse anti Human CD284
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00206
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names HTA125
Calculated MW 95680 Da
Additional Information
Other Species Rh,Gp,Pig,Dog
Purification Purified IgG prepared by affinity chromatography on Protein G.
Immunogen Ba/F3 cell line expressing TLR4 (CD284).
Shelf Life 18 months from date of despatch.
Gene ID 7099
Other Names Toll-like receptor 4, hToll, CD284, TLR4
Target/Specificity Mouse anti-Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti-Human CD284 antibody, clone HTA125 (ABD12360EL ) is recommended for functional assays.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD284 Antibody, clone HTA125 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll like receptor 4.
J. Exp. Med. 189: 1777-1182. 2. Jiang, Q. et al. (2000) Lipopolysaccharide induces physical proximity between CD14 and Toll like receptor 4 (TLR4) prior to nuclear translocation of NF-Kappa B.
J. Immunol. 165: 3541 - 3544. 3. Yang, S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture.
Infect. Immun. 69: 2045-2053. 5. Kawahara, T. et al. (2001) Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.
Infect. Immun. 69: 4382 - 4839. 6. Devaney, J.M. (2003) Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4.
FEBS Lett. 544:129-32. 7. de Kleer, I. (2010) CD30 Discriminates Heat Shock Protein 60-Induced FOXP3+CD4+ T Cells with a Regulatory Phenotype.
J Immunol. 185(4):2071-9. 8. Bieback, K. et al. (2002) Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling.
J Virol. 76: 8729-36. 9. Brännström, K. et al. (2009) The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling.
Infect Immun. 77: 1144-54. 10. Baumgarten, G. et al. (2001) In vivo expression of proinflammatory mediators in the adult heart after endotoxin administration: the role of toll-like receptor-4.
J Infect Dis. 183: 1617-24. 11. Cuschieri, J. et al. (2006) Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activation.
J Leukoc Biol. 80: 1289-97. 12. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.
Infect Immun. 70: 6688-96. 13. Medvedev, A.E. et al. (2001) Induction of tolerance to lipopolysaccharide and mycobacterial components in Chinese hamster ovary/CD14 cells is not affected by overexpression of Toll-like receptors 2 or 4.
J Immunol. 167: 2257-67. 14. Pioli, P.A. et al. (2007) Estradiol attenuates lipopolysaccharide-induced CXC chemokine ligand 8 production by human peripheral blood monocytes.
J Immunol. 179: 6284-90. 15. Sugawara, S. et al. (2000) Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingivalis leading to lipopolysaccharide hyporesponsiveness.
J Immunol. 165: 411-8. 16. Lindsay, J.O. et al. (2006) Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease.
Gut. 55: 348-55. 17. Komori, H. et al. (2012) a1-Acid Glycoprotein Up-regulates CD163 via TLR4/CD14 Protein Pathway: POSSIBLE PROTECTION AGAINST HEMOLYSIS-INDUCED OXIDATIVE STRESS.
Biol Chem. 287: 30688-700. 18. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. 19. Sels, J.W. et al. (2012) Fractional flow reserve is not associated with inflammatory markers in patients with stable coronary artery disease.
PLoS One. 7: e46356. 20. Prokhorenko, I. et al. (2012) Toll-like receptor 4 in phagocytosis of Escherichia coli by endotoxin-activated human neutrophils in whole blood
Critical Care 16: P80 21. Mazzucchelli, I. et al. (2015) Expression and function of toll-like receptors in human circulating endothelial colony forming cells.
Immunol Lett. 168 (1): 98-104. 22. Garbe, K. et al. (2012) Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.
Hum Immunol. 73 (5): 493-7. 23. Zwolak, A. et al. (2016) Metformin Changes the Relationship between Blood Monocyte Toll-Like Receptor 4 Levels and Nonalcoholic Fatty Liver Disease-Ex VivoStudies.
PLoS One. 11 (3): e0150233. 24. Zwolak A et al. (2015) Hyperreactivity of Blood Leukocytes in Patients with NAFLD to Ex Vivo Lipopolysaccharide Treatment Is Modulated by Metformin and Phosphatidylcholine but Not by Alpha Ketoglutarate.
PLoS One. 10 (12): e0143851. 25. Xu, H. et al. (2015) Type 3 innate lymphoid cell depletion is mediated by TLRs in lymphoid tissues of simian immunodeficiency virus-infected macaques.
FASEB J. 29 (12): 5072-80. 26. Blagitz, M,G. et al. (2015) Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis
Pesquisa Veterinária Brasileira. 35 (1): 1-5.

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Cat# ABD12360
(40 western blots)
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