|Application ||WB, FC, IP|
|Calculated MW||95680 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Immunogen||Ba/F3 cell line expressing TLR4 (CD284).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Toll-like receptor 4, hToll, CD284, TLR4|
|Target/Specificity||Mouse anti-Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti-Human CD284 antibody, clone HTA125 (ABD12360EL ) is recommended for functional assays.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD284 Antibody, clone HTA125 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll like receptor 4.J. Exp. Med. 189: 1777-1182. 2. Jiang, Q. et al. (2000) Lipopolysaccharide induces physical proximity between CD14 and Toll like receptor 4 (TLR4) prior to nuclear translocation of NF-Kappa B. J. Immunol. 165: 3541 - 3544. 3. Yang, S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture. Infect. Immun. 69: 2045-2053. 5. Kawahara, T. et al. (2001) Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.Infect. Immun. 69: 4382 - 4839. 6. Devaney, J.M. (2003) Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4. FEBS Lett. 544:129-32. 7. de Kleer, I.
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