|Calculated MW||85731 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Mouse transformed endothelioma cell line t.end1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, CD71, Tfrc, Trfr|
|Target/Specificity||Rat anti-Mouse CD71 antibody, clone 8D3 recognizes mouse CD71, a 763 amino acid ~95 kDa single pass type II transmembrane glycoproteincell surface, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.Rat anti-Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.Rat anti-Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kisselet al.1988).|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD71 Antibody, clone 8D3 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kissel, K. et al. (1998) Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibody.Histochem cell Biol. 110: 63-72. 2. Lee, H.J. et al. (2000) Targeting rat anti-mouse transferin receptor monoclonal antibodies through blood-brain barrier in mouse.J. Pharmacol Exp Ther. 292: 1048-52. 3. Zhang, Y. et al. (2004) Intravenous RNA interference gene therapy targeting the human epidermal growth factor receptor prolongs survival in intracranial brain cancer.Clin Cancer Res. 10 (11): 3667-77. 4. Cabezón I et al. (2015) Trafficking of Gold Nanoparticles Coated with the 8D3 Anti-Transferrin Receptor Antibody at the Mouse Blood-Brain Barrier.Mol Pharm. 12 (11): 4137-45. 5. Lee, H.J. et al. (2002) Imaging gene expression in the brain in vivo in a transgenic mouse model of Huntington's disease with an antisense radiopharmaceutical and drug-targeting technology.J Nucl Med. 43 (7): 948-56. 6. Manich, G. et al. (2013) Study of the transcytosis of an anti-transferrin receptor antibody with a Fab' cargo across the blood-brain barrier in mice.Eur J Pharm Sci. 49 (4): 556-64. 7. Sehlin, D. et al. (2016) Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer's disease.Nat Commun. 7: 10759.1. Jones AR & Shusta EV (2007) Blood-brain barrier transport of therapeutics via receptor-mediation.Pharm Res. 24 (9): 1759-71.2. Pardridge WM (2007) Blood-brain barrier delivery of protein and non-viral gene therapeutics with molecular Trojan horses.J Control Release. 122 (3): 345-8.3. Boado RJ et al. (2009) Engineering and expression of a chimeric transferrin receptor monoclonal antibody for blood-brain barrier delivery in the mouse.Biotechnol Bioeng. 102 (4): 1251-8.
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