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Anti Chlamydia LPS Antibody, clone CF 6J12

Mouse Anti-Bacterial Monoclonal Antibody

  • IF - Anti Chlamydia LPS Antibody, clone CF 6J12  ABD12367
    Published customer image:Mouse anti Chlamydia LPS antibody, clone CF 6J12 used for the detection of Chlamydia LPS in aortic lesions of Chlamydia infected mive by immunofluorescence.Image caption:1a. Agarose gel electrophoresis of PCR products (first stage, lanes 2–6; second stage, lanes 8–12). Lanes 2 and 3 using genomic DNA as a template from two Apobtm2SgyLdlrtm1Her/J mice infected with Cpn bacteria; lanes 5 and 6 using genomic DNA from two non-infected Apobtm2SgyLdlrtm1Her/J mice; lanes 8, 9, 11 and 12 using first-stage PCR products 2, 3, 5 and 6 as a template, respectively. Lanes 1 and 7 show the Phi174 DNA/HaeIII maker . 1b. Chlamydia LPS antibody (MCA 2718, AbD serotec) stained lesion sites in aortic sinus. Chlamydia LPS antibody (MCA 2718, AbD serotec) was used as the first antibody (10 mg/ml), anti-mouse IgG-FITC, developed in sheep, was used as a second antibody. Green represents Chlamydia LPS and blue represents cell nuclei stained with 4',6-diamidino-2-phenylindole (Vector Lab, Peterborogh, UK) [N = 6 mice].From: Xia M, Chen D, Endresz V, Faludi I, Szabo A, Gonczol E, et al. Immunization of Chlamydia pneumoniae -Infected Apobtm2SgyLdlrtm1Her/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development. PLoS ONE 8: e81056.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bacteria
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CF 6J12
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Elementary bodies from C. trachomatis strain SAF2.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Chlamydia LPS antibody, clone CF 6J12 recognizes a genus specific epitope within Chlamydia lipopolysaccharide (LPS).LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesotaorNeisseria gonorrhoeae.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Chlamydia LPS Antibody, clone CF 6J12 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12367
(40 western blots)
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