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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Human CD44 Antibody, clone 156-3C11    

Anti Human CD44 Antibody, clone 156-3C11

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti Human CD44 Antibody, clone 156-3C11  ABD12374
    Published customer image:Mouse anti Human CD44 antibody, clone 156-3C11 used for for the determination of CD44 expression on human small cell lung cancer cell tumors by immunohistochemistry.Image caption:Decreased survival of SCLC patients with moderate CEA expression levels. TMA of SCLC patients was immunohistochemically stained against CEA. Three patient groups were discriminated: (D) no CEA expression (CEA-), (E) moderate CEA expression (CEA+) and (F) strong CEA expression (CEA++). Kaplan Meier survival curves show significantly decreased median survival of patients with moderate CEA levels compared to patients with (A) negative (log-rank test, p?=?0.0054) or (B) strong CEA levels (log-rank test, p?=?0.0002), but no significant difference between median survival of patients (C) with negative compared to strong CEA levels (log-rank test, p?=?0.1016). In parallel a TMA of SCLC was immunohistochemically stained against CD44. Two patient groups were discriminated: (H) CD44 negative (CD44-) and (I) CD44 positive (CD44+). Kaplan Meier survival curve shows no statistical different median survival of patients (G) with negative CD44 levels compared with patients with positive CD44 levels (log-rank test, p?=?0.7). Scale bar?=?200 μm.From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al. Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.PLoS ONE 9(4): e92327.
  • IHC - Anti Human CD44 Antibody, clone 156-3C11  ABD12374
    Published customer image:Mouse anti Human CD44 antibody, clone 156-3C11 used for for the determination of CD44 expression on human small cell lung cancer cell (OH-1) tumors in mice by immunohistochemistry.Image caption:Spontaneous OH-1 metastases express CEA. Primary OH-1 tumor as well as spontaneous OH-1 lung and bone metastases were positively stained for CD44, CEA, EpCAM and PGP9.5 (red?=?positive cells). Metastases are indicated by red asterisks. Scale bar: 50 μm for all panels.From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al. Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.PLoS ONE 9(4): e92327.
  • FC - Anti Human CD44 Antibody, clone 156-3C11  ABD12374
    Published customer image:Mouse anti Human CD44 antibody, clone 156-3C11 used for for the determination of CD44 expression on small cell lung cancer cells by flow cytometry.Image caption:SCLC cell line OH-1 binds to selectins and expresses CEA. OH-1 cells, as a cell line of neuroectodermal origin, are positive for NCAM and EpCAM, which could be detected by flow cytometry analysis and can thus serve as a marker for these cells when grown in mice. Cells show binding to E- and P-selectin fusion protein. The glycosylation motif sialyl Lewis A, a binding partner recognized by selectins, could be detected with the CA19-9 antibody. The cell line OH-1 exhibits several known proteins which known to be protein backbones for selectin binding carbohydrate residues such as PSGL-1, MUC18, CD44 and CEA. Isotype controls are shown as dotted lines.From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al. Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.PLoS ONE 9(4): e92327.
  • WB - Anti Human CD44 Antibody, clone 156-3C11  ABD12374
    Published invetstigator image:Mouse anti Human CD44 antibody, clone 156-3C11 used for the evaluation of CD44 expression on JIMT-1 breast cancer cell lysate by western blotting.Image caption:Mammosphere propagation of cell lines changed the mRNA and protein expression of CD44 isoforms. A. Breast cancer cell lines grown as monolayer cells or mammospheres were analyzed for mRNA expression of CD44 isoforms using q-RT-PCR. In general, the gene expression of the alternatively spliced variants CD44v2-v10, CD44v3-v10, CD44v8-v10 increased and the expression of CD44S decreased for cells cultured as mammospheres. B. Western blot analysis (CD44 antibody, clone 156-3C11) of the breast cancer cell line JIMT-1 confirmed changes in expression of CD44 variants for cells propagated as mammospheres.From: Olsson, E. et al. CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers.BMC Cancer. 2011 Sep 29;11:418.
  • WB - Anti Human CD44 Antibody, clone 156-3C11  ABD12374
    Western blot analysis of Hela whole cell lysate probed with Mouse anti Human CD44 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, FC, IP
Primary Accession P16070
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 156-3C11
Calculated MW 81538 Da
Additional Information
Other Species Baboon,African Green Monkey
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Stimulated human leucocytes.
Shelf Life 18 months from date of despatch.
Gene ID 960
Other Names CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4
Target/Specificity Mouse anti Human CD44 antibody, clone 156-3C11 recognizes human Phagocytic glycoprotein 1 also known as CD44, HCAM or CD44s. CD44 is a ~90 kDa single pass type I transmembrane glycoprotein. Various isoforms of CD44 exist due to differential expression of exon products form the membrane proximal region of the extracellular domain. Mouse anti Human CD44 antibody, clone 156-3C11 recognizes the ~90 kDa standard form lacking any of the alternative spliced products, the clone is expected to recognize all isoforms of CD44. CD44 is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, defined as a protease resistant epitope on the CD44 molecule (CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al).CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration (Lesleyet al.1990). CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing (Shimizuet al.1989). CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (East and Hart 1993).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD44 Antibody, clone 156-3C11 is for research use only and not for use in diagnostic or therapeutic procedures.
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