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>   home   >   Products   >   Primary Antibodies   >   Anti Rat CD25 Antibody, clone OX-39    

Anti Rat CD25 Antibody, clone OX-39

Mouse Anti-Rat Monoclonal Antibody

     
  • FC - Anti Rat CD25 Antibody, clone OX-39  ABD12381
    Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin
  • IF - Anti Rat CD25 Antibody, clone OX-39  ABD12381
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • IF - Anti Rat CD25 Antibody, clone OX-39  ABD12381
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
  • IHC - Anti Rat CD25 Antibody, clone OX-39  ABD12381
    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power
  • FC - Anti Rat CD25 Antibody, clone OX-39  ABD12381
    Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P26897
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names OX-39
Calculated MW 30557 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Stimulated Rat T cells
Shelf Life 18 months from date of despatch.
Gene ID 25704
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, CD25, Il2ra
Target/Specificity Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Patersonet al.1987).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD25 Antibody, clone OX-39 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12381
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