|Application ||WB, IHC-P, IF|
|Calculated MW||18358 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Purified GST-LMO2 fusion protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2, LMO2, RBTN2, RBTNL1, RHOM2, TTG2|
|Target/Specificity||Mouse anti Human LMO2 antibody, clone 1A9-1 recognizes the human Rhombotin-2 also known as LMO2 or T-cell translocation protein 2. LMO2 is a 158 amino acid ~24kD transcription factor containing 2 LIM domains that interacts with DNA binding proteins such as TAL1/SCLandGATA1. In early development, LMO2 is expressed in the central nervous system (Herberthet al.2005) and a wide range of tissue, and in adults in myeloid and erythroid precursors, in normal germinal centre B-cells, endothelial cells and parts of the brain (Natkunamet al.2006). LMO2 plays a vital role in early stages of haematopoiesis and in regulating yolk-sac erythropoiesis in mice (Kathleenet al.2005). Studies suggest that it may also play a role in angiogenesis (Yamadaet al.2002).A major cause of T-cell acute lymphoblastic leukemia (T-ALL) is the aberrant activation of the TAL1/SCL, LMO1/2, and NOTCH1 oncogenes (Ferrandoet al.2004). Activation of the Notch signalling pathway by mutations in either the gamma-secretase or PEST domains are found in more than 50% of all T-ALL, including LMO2-induced T-ALL, and studies using mouse models, have show that activation of Notch co-operates with Scl/Lmo1 in inducing T-ALL (Wenget al.2004). Aberrant expression of LMO2 has also been linked to certain types of B-cell lymphoma (Natkunamet al.2008) and prostate cancer, with overexpression possibly linked to cancer progression (Maet al.2007).Mouse anti human LMO2, clone 1A9-1 has been used for the detection of LMO2 by immunohistochemistry on formalin fixed, paraffin embedded tissues and by Western blotting (Natkunamet al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human LMO2 Antibody, clone 1A9-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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