|Application ||WB, E|
|Calculated MW||29274 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant human soluble CD154 conjugated to BSA.|
|Shelf Life||18 months from date of despatch.|
|Other Names||CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP|
|Target/Specificity||Mouse anti Human CD154 (Soluble) antibody, clone 2A3 recognizes the soluble form of CD154, otherwise known as CD40 Ligand/TRAP, a ~32 kDa glycoprotein and member of the TNF family, which is specifically expressed on activated, but not resting, CD4+ T cells, dendritic cells (DCs), B cells and macrophages. CD154 binds CD40, and acts as a key co-stimulatory molecule through the activation of antigen-presenting cells, and as a regulator of the generation and functional properties of Th1 helper cells. CD154 exists in both a membrane-bound and soluble form, the latter of which is generated through proteolytic processing by a matrix metalloproteinase (MMP), in activated cells.The binding of CD154 with its receptor CD40 is vital for many biological processes, including Ig class switching, germinal centre formation and the survival of CD40+ B cells, B cell proliferation in the absence of co-stimulus, the production of IgE in the presence of interleukin-4 (IL-4), and also in the production of pro-inflammatory cytokines, MMPs, prostaglandins and adhesion molecule upregulation, in non-immune cells. Mouse anti Human CD154 (Soluble) antibody, clone 2A3 recognizes the soluble form of the protein.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human CD154 (soluble) Antibody, clone 2A3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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