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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse Ly-6A/E Antibody, clone D7    

Anti Mouse Ly-6A/E Antibody, clone D7

Rat Anti-Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05533
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names D7
Calculated MW 14377 Da
Additional Information
Purification Purified IgG prepared from ascites or tissue culture supernatant
Immunogen IL-2-dependent mouse T cell line CTL-L.
Shelf Life 18 months from the date of despatch
Gene ID 110454
Other Names Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly6a, Ly6
Target/Specificity Rat anti Mouse Ly-6A/E antibody, clone D7 recognises members of the lymphocyte activation protein-6 (Ly-6) multigene family. Specifically, it recognises Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of Sodium Azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Ly-6A/E Antibody, clone D7 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12411
(40 western blots)
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