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Anti Human CD40 Antibody, clone 3B2

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human CD40 Antibody, clone 3B2  ABD12412
    Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Undiluted serum and plasma samples are shown green and orange respectively.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25942
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3B2
Calculated MW 30619 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen CD40-Fc.
Shelf Life 18 months from date of despatch.
Gene ID 958
Other Names Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40, CD40, TNFRSF5
Target/Specificity Mouse anti Human CD40 antibody, clone 3B2 recognizes human CD40, a type I transmembrane glycoprotein receptor and member of the TNF-receptor superfamily, primarily expressed by B cells and professional antigen-presenting cells (APCs), which plays a vital role in T cell-B cell interactions and in germinal centre B cell selection. CD40 is a co-stimulatory molecule which mediates its immunoregulatory effects through interaction with its ligand CD154 (CD40L, gp39), playing a role in B cell proliferation and memory development, Ig class switching, activation of APCs and subsequent cytokine production, and the expansion of antigen-specific CD4+ T cells.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD40 Antibody, clone 3B2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12412
(40 western blots)
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