|Application ||WB, E|
|Calculated MW||85811 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Highly purified protective antigen of Bacillus anthracis.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Protective antigen, PA, Anthrax toxins translocating protein, PA-83, PA83, Protective antigen PA-20, PA20, Protective antigen PA-63, PA63, pagA, pag|
|Target/Specificity||Mouse anti Anthrax Protective Antigen antibody, clone C3 recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. Mouse anti Anthrax Protective Antigen antibody, clone C3 does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensisorToxoplasma gondii. The antibody recognizes both the 83kDa and 63kDa truncated form of PA.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Anthrax Protective Antigen Antibody, clone C3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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