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>   home   >   Products   >   Primary Antibodies   >   Anti Ricin A Chain Antibody, clone RA999    

Anti Ricin A Chain Antibody, clone RA999

Mouse Anti-Plants Monoclonal Antibody

     
  • WB - Anti Ricin A Chain Antibody, clone RA999  ABD12476
    Published customer image:Mouse anti ricin A chain antibody, clone RA999 used for the detection of wild type ricin following incubation with HEK293 cells in vitro by western blotting under non-reducing conditions.Image caption:DHF ricin and IHF ricin show decreased binding to the cell surface and increased degradation in low pH vesicles. (A) HEK293 cells after 3 hours incubation with wild-type ricin, IHF ricin or DHF ricin run on SDS/PAGE under non-reducing conditions. A representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. RTA wt is marked as 1, other results are relative to this control. (B) Cells were incubated with wild-type ricin, IHF ricin or DHF ricin for 30 min at 0°C. Binding was measured as described in Methods. A representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. RTA wt is marked as 1, other results are relative to this control. (C) Coomasie Blue-stained 12% SDS/PAGE gels showing the stability of 500 ng of wild-type ricin, and modified ricin IHF and DHF after 30 min incubation at 0°C. (D) Cells were treated with or without bafilomycin A1 (0.1 µM) and with wild-type ricin, IHF ricin or DHF ricin. The amounts of ricin that remain in the cell after degradation was analyzed after SDS-PAGE run under non-reducing conditions (see Methods). Representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. Ricin wt, ricin IHF and ricin DHF in each graph are marked as 1, other results are relative to this control. All degraded forms of ricin were analyzed. (E) Tyrosine sulfated wild-type ricin sulf-1, and modified ricin sulf-1: IHF or DHF in cells after 3 hours incubation with Na235SO4 and further 3 h incubation with the toxins, run on SDS/PAGE under non-reducing conditions. Control experiments revealed equal total sulfation of proteins in cell lysates. A representative autoradiogram is shown.From: Sokołowska I, Piłka ES, Sandvig K, Węgrzyn G, Słomińska-Wojewódzka M. Hydrophobicity of protein determinants influences the recognition of substrates by EDEM1 and EDEM2 in human cells.BMC Cell Biol. 2015 Feb 6;16:1.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P02879
Reactivity Plants
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names RA999
Calculated MW 64091 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Full length Ricin A (RCA60) from Ricinus communis.
Shelf Life 18 months from date of despatch.
Gene ID 8261245
Other Names Ricin, Ricin A chain, 3.2.2.22, rRNA N-glycosidase, Linker peptide, Ricin B chain, RICI
Target/Specificity Mouse anti Ricin A Chain antibody, clone RA999 recognizes the A chain of the ricin toxin (RCA60), a 60kDa glycoprotein. Mouse anti Ricin A Chain antibody, clone RA999 also cross-reacts with RCA120.The ricin toxin consists of two chains, A and B, covalently linked by a disulfide bond. The A chain acts as a glycosidase that removes an adenine residue from the 28S rRNA. The area from which the adenine is removed is essential for binding of elongation factors, destroying it leaves the ribosome inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, much faster than the cell can replace them, meaning that a single ricin A chain can kill a cell.The B chain binds to complex carbohydrates on the cell surface to facilitate entry of the A chain into the cell. Without the B chain, the A chain has very low toxicity.Ricin is easily extracted from the castor bean (Ricinus communis), and the lethal dose for humans is very small. It is toxic when inhaled, ingested or injected and there is no known antidote, although a vaccine has been developed. Treatment is symptomatic and supportive, with long term organ damage likely in survivors. The ease of extraction and distribution makes it a potential candidate for biological warfare although it is less stable than other agents, such as anthrax.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Ricin A Chain Antibody, clone RA999 is for research use only and not for use in diagnostic or therapeutic procedures.
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