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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human CA125 Antibody, clone X325    

Anti Human CA125 Antibody, clone X325

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti Human CA125 Antibody, clone X325  ABD12485
    Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X75 as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X325 as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma sample at 1:2 is shown
  • E - Anti Human CA125 Antibody, clone X325  ABD12485
    Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X325 as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X75 as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma sample at 1:2 is shown
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8WXI7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names X325
Calculated MW 2353428 Da
Additional Information
Purification Purified IgG prepared by ion exchange chromatography
Immunogen CA125 antigen purified from human ovarian carcinoma.
Shelf Life 18 months from date of despatch.
Gene ID 94025
Other Names Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125, MUC16, CA125
Target/Specificity Mouse anti Human CA125 antibody, clone X325 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X325 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustadet al.2002). Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CA125 Antibody, clone X325 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12485
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