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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human H-Ras Antibody, clone H-Ras-03    

Anti Human H-Ras Antibody, clone H-Ras-03

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human H-Ras Antibody, clone H-Ras-03  ABD12492
    Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human H-Ras antibody followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01112
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names H-Ras-03
Calculated MW 21298 Da
Additional Information
Other Species M,Rat
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Synthetic peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras.
Shelf Life 18 months from date of despatch.
Gene ID 3265
Other Names GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Target/Specificity Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburnet al.1990).H-Ras,N-RasandK-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarenaet al.2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Grippet al.2006).Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluotoet al.2011).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human H-Ras Antibody, clone H-Ras-03 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12492
(40 western blots)
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