|Application ||WB, IF, E, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti V5-Tag antibody, clone SV5-Pk4 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk4 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti V5-Tag Antibody, clone SV5-Pk4 is for research use only and not for use in diagnostic or therapeutic procedures.|
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