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>   home   >   Products   >   Primary Antibodies   >   Anti Rat CD68 Antibody, clone ED1    

Anti Rat CD68 Antibody, clone ED1

Mouse Anti-Rat Monoclonal Antibody

     
  • IHC - Anti Rat CD68 Antibody, clone ED1  ABD12511
    Published customer image:Mouse anti Rat CD68 antibody, clone ED-1 used for the identification of CD68 expressing cells in rat kidney by immunohistochemistryImage caption:ED-1 stain of kidney tissue. The groups are (A) control, (B) EG-exposed, (C) EG+STS-exposed, (D) EG+SC-exposed, (E) EG+SS-exposed animal groups. The ED-1 stain was markedly increased in EG-exposed animals but decreased in all treatment groups. The significance levels are with reference to the EG group. The data are means ± SD from 7–8 animals per group. ***p < 0.001.From: Bijarnia RK, Bachtler M, Chandak PG, van Goor H, Pasch A Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats. PLoS ONE 10(4): e0124881.
  • IF - Anti Rat CD68 Antibody, clone ED1  ABD12511
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power
  • IF - Anti Rat CD68 Antibody, clone ED1  ABD12511
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power
  • IHC-P - Anti Rat CD68 Antibody, clone ED1  ABD12511
    Published customer image:Mouse anti Rat CD68 antibody, clone ED1 used for the identification of macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:(A) Representative sections from the ventral prostate lobe of Dunning tumor-bearing and control rats stained to visualize CD68+ macrophages (brown, 200X magnifications, T; tumor). (B) Volume density of CD68+ macrophages in the tumor-adjacent prostate tissue and in controls. a; significantly different than controls, b; large G tumors significantly different than small G tumors, and c; significantly different than corresponding tumor at day 7, P<0.05, n = 5–13. (C) Scatterplot of the volume density of CD68+ macrophages in the tumor-bearing organ plotted against tumor weight (correlation coefficients are given in the result text).From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A Adaptive Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.PLoS ONE 10: e0141601.
  • IHC-P - Anti Rat CD68 Antibody, clone ED1  ABD12511
    Published customer image:Mouse anti Rat CD68 antibody, clone ED1 used for the detection of CD68 expressing macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Renal ED-1 immunostaining of the rats treated for 28 days: sham (A), losartan (B), leptin (C) and leptin plus losartan (D). ED-1-positive cell quantification (in cells/area) (E). Magnification of the leptin-treated rats ED-1 positive cell area. G = glomeruli; arrowheads = ED-1-positive cells; bar = 100 µm. *p
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names ED1
Additional Information
Other Species B
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Rat spleen cells
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of 90-110 KDa, also known as rat CD68 (Dijkstraet al.1985).The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseauxet al.1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstraet al.1985).The expression of ED1 antigen being predominantly cytoplasmic (Dijkstraet al.1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD68 Antibody, clone ED1 is for research use only and not for use in diagnostic or therapeutic procedures.
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