|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Rat Spleen cell homogenate.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstraet al.1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabrieket al.2007).Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstraet al.1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbeet al.1990).Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whitelandet al.|
|Preservative & Stabilisers||0.09% Sodium Azide, 1% Bovine Serum|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Rat CD163 Antibody, clone ED2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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