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>   home   >   Products   >   Primary Antibodies   >   Anti Rat CD163 Antibody, clone ED2    

Anti Rat CD163 Antibody, clone ED2

Mouse Anti-Rat Monoclonal Antibody

  • IF - Anti Rat CD163 Antibody, clone ED2  ABD12512
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power
  • IHC - Anti Rat CD163 Antibody, clone ED2  ABD12512
    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power
  • FC - Anti Rat CD163 Antibody, clone ED2  ABD12512
    Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163
  • FC - Anti Rat CD163 Antibody, clone ED2  ABD12512
    Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE
  • IHC - Anti Rat CD163 Antibody, clone ED2  ABD12512
    Published customer image:Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraqffin embedded tissue sections and and flow cytometry on isolated cells.Image caption:CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining . Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.PLoS ONE 8(1): e53312.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names ED2
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Rat Spleen cell homogenate.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstraet al.1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabrieket al.2007).Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstraet al.1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbeet al.1990).Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whitelandet al.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD163 Antibody, clone ED2 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12512
(40 western blots)
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