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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti MBP (aa82-87) Antibody, clone 12    

Anti MBP (aa82-87) Antibody, clone 12

Rat Anti-Bovine Monoclonal Antibody

     
  • WB - Anti MBP (aa82-87) Antibody, clone 12  ABD12529
    Published customer image:Rat anti MBP antibody used for the evaluation of myelin basic protein expression in mouse Schwann cells using immunofluorescence and western blotting Image caption:Inverse correlation of MBP and sncRNA715 in primary Schwann cells. A, Primary Schwann cells derived from sciatic nerves of P3 Wistar rats were cultured in non-differentiating or differentiating (+NRG1 +dbcAMP) conditions. MBP protein can only be detected by immunocytochemistry in differentiated Schwann cells. Scale bar represents 50μm. B, Western Blots of undifferentiated and differentiated primary Schwann cells show MBP protein only present in differentiated Schwann cells. CNP is expressed in both maturation stages of primary Schwann cells. GAPDH serves as loading control. C, MBP and sncRNA715-specific RT-PCR on RNA extracted from undifferentiated or differentiated primary Schwann cells. Mbp mRNA is present at both differentiation states while sncRNA715 is detectable in undifferentiated and hardly in differentiated Schwann cells. SnoRNA135 and G6pdh mRNA were used as loading controls.From: Müller C, Hochhaus NM, Fontana X, Luhmann HJ, White R SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.PLoS ONE 10(8): e0136900.
  • IF - Anti MBP (aa82-87) Antibody, clone 12  ABD12529
    Published customer image:Rat anti MBP antibody, clone 12 used for the detection of murine myelin basic protein by immunofluorescence .Image caption:P0 localization in myelinated fibers of DRG cultures and in human sciatic nerves. (A) Confocal images (z-series projections) of P0 and MBP coimmunofluorescence in myelinated mouse DRG cultures. Overlay appears yellow. DRG cultures of six control embryos were analyzed, and a representative P0-positive fiber is shown. White dashed lines delineate MBP signal that is apparently restricted to internodes, whereas P0 signal extends further between two internodes. Scale bar = 5 µm. (B–C) Coimmunofluorescence of total neurofascins (NFasc, red) and P0 (B) or of NFasc186 , Contactin , and P0 (C) on longitudinal cryosections of adult human peripheral nerves. Median, tibial, and/or sciatic nerves of three human individuals were analyzed and representative pictures of sciatic nerves are shown. P0 was abundant in all paranodal/nodal regions we observed. Z-series projections and single optical sections are shown. Asterisks mark the position (lateral dimension) of the node of Ranvier. Scale bars = 5 μm in (B) and 10 μm in (C).From: Brügger V, Engler S, Pereira JA, Ruff S, Horn M, Welzl H, et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.PLoS Biol 13(9): e1002258.
  • WB - Anti MBP (aa82-87) Antibody, clone 12  ABD12529
    Published customer image:Rat anti MBP antibody used for the evaluation of myelin basic protein expression in mouse sciatic nerve lysates by western blotting Image caption:Inverse correlation of MBP and sncRNA715 in the sciatic nerve. A&B, The sciatic nerve was lysed from mice at postnatal day 1, 4 and 9 and myelin proteins as well as sncRNA715 expression was analyzed by Western blotting (A) and qPCR (B), respectively. MBP and CNP Western blots show increasing levels in differentiating sciatic nerves (A) while sncRNA715 levels decrease during differentiation, P-values P4: 0,0313, P9: 0,0313 (B, log2 values are plotted, sncRNA715 levels at P4 and P9 were quantified relative to P1 using snoRNA135 as a reference gene). Number of experiments (n) are indicated and bar graphs represent mean values ± s.e.m. (Wilcoxon signed-rank test, *P< 0.05, GraphPad Prism5 was used for statistical analysis).From: M&uuml;ller C, Hochhaus NM, Fontana X, Luhmann HJ, White R SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.PLoS ONE 10(8): e0136900.
  • IF - Anti MBP (aa82-87) Antibody, clone 12  ABD12529
    Published customer image:Rat anti MBP antibody, clone 12 used for the detection of myelin basic protein by immunofluorescence on formalin fixed, paraffin embedded tissue sections.Image caption:Demyelination/remyelination and decreased P0 expression in dKO mice. (A) Electron micrographs of ultrathin cross sections of control, dKO, H1HTZ, H2HTZ, H1KO, and H2KO sciatic nerves, at 8 wk post-tamoxifen, and percentage of demyelinated/remyelinated axons (3 animals per group, at least 700 axons counted per mouse), identifying a demyelination/remyelination phenotype in dKO sciatic nerves. Asterisks indicate demyelinated axons and “M” macrophages. (B) CD68 immunofluorescence in longitudinal cryosections of control and dKO sciatic nerves labeled with DAPI (blue = nuclei) showing increased presence of macrophages in dKO sciatic nerves, consistent with the demyelination phenotype. Arrows indicate macrophages. (C) In situ hybridization of P0 on longitudinal cryosections of control and dKO sciatic nerves identifying a reduction of P0 at the transcript level at 5 wk post-tamoxifen, before the onset of demyelination and when macrophages are not present in the nerve. Pictures on the right are magnifications of black boxes depicted on the left. Black arrows show SC nuclei. (D) Western blot of P0 and quantification normalized to GAPDH (loading control) in sciatic nerve lysates of control and dKO mice at 8 wk post-tamoxifen (3 mice per group), showing reduced P0 protein levels in dKO sciatic nerves. (E) Confocal images of MBP and P0 coimmunofluorescence in paraffin cross sections of dKO mice at 8 wk post-tamoxifen, showing reduced P0 levels in most myelin rings, while MBP levels remain high. Nuclei are labeled in blue by DAPI. A single optical section is shown. White arrows indicate MBP positive/P0 negative myelin rings, the yellow arrowhead shows an MBP/P0 double negative SC, and blue arrowheads MBP/P0 double positive myelin rings. Three animals per group were used for each experiment. P-values (two-tailed unpaired (A) or paired (D) Student&#39;s t test): * = p < 0.05, ** = p < 0.01, *** = p < 0.001, error bars = SEM.From: Br&uuml;gger V, Engler S, Pereira JA, Ruff S, Horn M, Welzl H, et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.PLoS Biol 13(9): e1002258.
  • IF - Anti MBP (aa82-87) Antibody, clone 12  ABD12529
    Published customer image:Rat anti MBP antibody, clone 12 used for the detection of Myelin basic protein in murine myelinating cell cultures using immunofluorescence.Image caption:Immunohistochemistry of transplanted neurospheres demonstrate that cyto-GFP labelled cells form early and mature myelinating oligodendrocytes. Cyto-GFP-expressing neurospheres were transplanted into a shiverer mouse 3, 7 or 15 days post-transplantation, and 10 µm thick frozen sections were cut and immunolabelled with antibodies to GFP and MBP. Low magnification image of a dorso-vental section of spinal cord, 15 days post-transplantation showing GFP (A) and MBP (B) immunostaining. Transplanted cells were located in both grey and white matter (dorsal columns are delineated by the dotted lines) and expressed MBP-positive myelin sheaths. C) A pre-myelinating cell in which multiple fine GFP positive processes emanate from a central cell body. The soma is also lightly stained with MBP, confirming the identity of the cell as that of the oligodendroglial lineage. MBP-positive myelin sheaths, belonging to a second cell are seen in the bottom left hand corner of the images. D) An early myelinating cell in which short MBP-positive profiles are present at the periphery of the GFP-positive soma. All images were obtained using epifluorescence Olympus microscope (FV10 ASW). Representative images from at least 30 separate experiments.From: Ioannidou K, Anderson KI, Strachan D, Edgar JM, Barnett SC Time-Lapse Imaging of the Dynamics of CNS Glial-Axonal Interactions In VitroandEx Vivo.PLoS ONE 7(1): e30775.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P02687
Reactivity Bovine
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 12
Calculated MW 18323 Da
Additional Information
Other Species M,Rb,Mammalian species,Rat,Gp,Sh,H,C,Pig
Immunogen Bovine MBP.
Shelf Life 18 months from date of despatch.
Other Names Myelin basic protein, MBP, 20 kDa microtubule-stabilizing protein, Myelin A1 protein, MBP
Target/Specificity Rat anti MBP antibody, clone 12 recognizes myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).Rat anti MBP antibody, clone 12 has been reported as being suitable for use in Western blotting (Glynnet al.1987).
Preservative & Stabilisers 0.1% Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti MBP (aa82-87) Antibody, clone 12 is for research use only and not for use in diagnostic or therapeutic procedures.
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